Scientific Journal of KubSAU

Polythematic online scientific journal
of Kuban State Agrarian University
ISSN 1990-4665
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100 kb

COMPARATIVE CHARACTERISTICS OF DI-AGNOSTIC LABORATORY TESTS FOR THE VIRUS PERITONITIS OF CATS

abstract 1471903031 issue 147 pp. 0 – 0 29.03.2019 ru 16
This article is devoted to the study of various laboratory tests for the diagnosis of viral peritonitis of cats, conducted in 2014-2018. The study was subjected to 278 cats of both sexes, different breeds and mongrel, of different ages, which were taken to the veterinary clinic «Vita» and the veterinary clinic «Noah's Ark» in Krasnodar. Analysis of the results showed that in the diagnosis and differential diagnosis of viral peritonitis of cats, the most resultant is a combination of studies of Globulin in serum and a test for antibodies to coronavirus, or a solid enzyme immunoassay (tIFA) on IgG to coronavirus. In addition to clinical symptoms, routine laboratory studies, instrumental studies for the diagnosis of viral cat peritonitis, it is necessary to use additional research methods, although many of them have a number of shortcomings in sensitivity and specificity
1207 kb

DEVELOPMENT AND JUSTIFICATION OF ZOOHYGIENIC ACTIVITIES TO IMPROVE THE PRODUCTIVITY OF BIRDS AND THEIR EPIZOOTIOLOGICAL SUSTAINABILITY IN CLOSED POULTRY HOUSES

abstract 1491905035 issue 149 pp. 0 – 0 31.05.2019 ru 18
The problems of creating an optimal microclimate in the closed workshops of poultry farms are relevant for the regions of the south of Russia and the Northern Caucasus, especially acute these problems are for Kabardino-Balkaria. The used zoohygienic measures and existing ventilation systems of closed poultry houses need to be improved and adapted to the local natural conditions. The purpose of the work was to research and develop effective ventilation systems for closed poultry houses in hot climates. Existing ventilation systems and zoohygienic conditions in closed-type poultry houses were studied and investigated. According to the results, the main disadvantages of the existing ventilation systems and their low efficiency were established. And on their basis, new technical solutions have been found to create more efficient ventilation systems for closed poultry houses that provide the optimal microclimate: temperature, humidity and pressure. With the active participation of the author, new technical solutions have been developed and proposed, for which two applications for obtaining patents have been prepared: one for a utility model - for a device for moistening and cooling wall ventilation ducts of poultry houses; the second is for the invention - on the method of erecting a cooling system and humidifying the ventilation panels of the supply. During their implementation, these new technical solutions allow creating such a microclimate, thanks to which optimal indicators of productivity and growth, uniformity, efficiency of feed and meat gain are achieved, without reducing the characteristics of the health and well-being of birds. Ventilation is becoming a major factor in microclimate control in closed houses and the epizootological resistance of birds
590 kb

ALLELE-SPECIFIC PCR FOR TYPING OMPH-TYPES OF PASTEURELLA MULTOCIDA AND SEARCING FOR NEW APPROACHES TO STUDYING OF PATHOGENICITY OF BACTERIAL ISOLATES

abstract 1471903033 issue 147 pp. 0 – 0 29.03.2019 ru 23
Pasteurella multocida is an important respiratory pathogen of cattle. OmpH Protein is a major protective antigen of bacteria has been well studied in avian strains. In the literature there are no data available for the study of a variety of sequence of this protein among isolates with cattle respiratory pathology. There have been described several genes associated with the virulence of the bacterium in respiratory disease of cattle, but none of the authors compared the frequency of detection of these genes with the pathogenicity for laboratory animals. The aim of our study was the development of allele-specific PCR to determine Omph-types of Pasteurella multocida and the search for new approaches to assess the pathogenicity of isolates of bacteria. Total amount of 83 isolates allocated from the lungs of calves with respiratory pathology was investigated. All isolates belonged to groups A or D (isolates 63 and 20, respectively). Among isolates of capsular serogroup A we revealed 6 types, most propagation types were A1 and A2. All isolates of capsular serogroup D were one omph- type. In 16 out of 23 farms there were identified isolates of only one omph-type, 4 - 2 types, 3 - three types. The frequency of gene hgbb - hemoglobin binding protein correlated with pathogenicity of isolates for white mice. The developed allele-specific PCR along with hgbb gene detection can be used for screening and studying the properties of antigen and circulating pathogenic isolates and selecting a candidate vaccine strains
277 kb

USING MILK UREA CONCENTRATION AS STRATEGY TO IMPROVE THE EFFICIENCY OF NITROGEN USE AND REDUCING NITROGEN LOSSES IN COMMERCIAL DAIRY HERDS

abstract 1491905032 issue 149 pp. 0 – 0 31.05.2019 ru 26
This prospective study was conducted on 10 commercial dairy herds, over one year on milk urea monitoring, determination of diets characteristics effects on MU concentration and on assessment of MU concentrations as a predictor of N utilization and urinary N excretion. Milk samples were collected twice every month and analyzed for urea concentration using a colorimetric procedure. Representative feed samples were also collected on the same day of milk collection. Feed samples were characterized and their concentrations of protein digestible in the intestine and net energy for lactation were calculated according to the French system as well as PDI requirements. Average of milk urea concentrations range is 25.0 - 32.0 mg/dl. A significant positive association (p
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SPECIAL ASPECTS OF DIAGNOSTICS, TREATMENT AND PREVENTION OF TURKEY SMALLPOX

abstract 1501906028 issue 150 pp. 0 – 0 28.06.2019 ru 30
Smallpox of turkey is a viral, slowly extending disease. It pathogenetically proceeds in a dermatotropic way, skin lesions are characterized primarily by two signs or focal necrotic focus centers or diphtheritic inflammation of mucous membranes, often of the upper respiratory tract. Etiological factor in smallpox are dermatotropic epitheliogenic viruses, the family of Avipoxvirus of Poxviridae. The purpose of this work was studying the features of clinic and the pathomorphological changes at smallpox at turkeys in the context of diagnostics methods and also generalization of treatment-and-prophylactic actions at this pathology. The diseased bird is the main source of infection. In addition, the smallpox virus particles can be spread by people providing care for the birds, indoor air, food, bedding material, excrement. Diagnosis of smallpox takes into account the symptoms; if an autopsy was performed, the result of a pathological pattern as well. Additionally, we can conduct a research of histological sections. Confirmation of diagnosis can be performed by a virological study and the blood serum. In accordance with the pathogenesis of smallpox, there is a classic skin form and diphtheritic. There may be a combination of the above manifestations of smallpox, therefore we can record the mixed form, in some cases, it may be atypical. Pathoanatomical changes come down to defeat a respiratory system of bodies, the upper airways, at the complicated forms there may be noted lesions in the internal organs. Laboratory methods include identification of the virus in the pathological material, as well as additional histological and virological studies using chicken embryos and bioassays. Treatment is difficult and not always effective. The main preventive measure against smallpox in turkey is vaccination, basic applied biologics are: Avivac-pox" strain "K", "Ospovat, Vectorman FP-MG". Prevention of smallpox includes the implementation of general veterinary and sanitary rules in conjunction with the mandatory special prevention. When planning measures to prevent the spread of smallpox, conditions are created to eliminate pathways of transmission of the pathogen – disinfection, disinsection and deratization, as well as vaccination of all susceptible livestock
4722 kb

AUTOMATED SYSTEM-COGNITIVE ANALYSIS OF THE EFFECTS OF PROBIOTICS IN DIETS ON BODY TYPE OF YOUNG BULLS

abstract 1461902033 issue 146 pp. 0 – 0 28.02.2019 ru 34
The article is devoted to the use of automated system-cognitive analysis for the study of probiotics for the growth of bulls. Moreover, as growth indicators we have not used live weight, but indices characterizing the shape and proportions of the body of animals. A detailed numerical example of solving the problem using real data is given
396 kb

OPTIMIZATION OF THE RELATION OF BIOLOGICALLY ACTIVE SUBSTANCES AND PROBIOTIC MICROFLORA IN THE RECIPE OF COMPLEX FEED CONCENTRATE

abstract 1481904032 issue 148 pp. 0 – 0 30.04.2019 ru 35
Providing the population with basic foodstuffs, sources of proteins, fats, carbohydrates, vitamins and other essential nutrients is one of the main issues of ensuring the security of the country, while the requirement for food safety is paramount. Production of feed additives (concentrates), which improve feed conversion, while being natural and safe for animal health, acquires the greatest importance in the conditions of intensification of agriculture and increasing the competitiveness of livestock farms. Probiotic microflora, which, in competition with pathogenic, displaces the latter from the gastrointestinal tract, can be considered as one of the effective methods of controlling undesirable microflora in the digestive tract. In world science, research is actively conducted in the direction of searching for effective probiotic cultures. A promising way to increase the effectiveness of the use of probiotic feed additives is their addition to prebiotics, for example, including vitamins (antioxidants) and mineral substances (selenium). The purpose of this work was to determine the optimal ratio of biologically active substances contained in the feed vitamin-mineral concentrate, and probiotic microflora contained in the feed probiotic additive Bacell-M, for the development of complex feed concentrate. Using the Harrington's desirability function, the optimum ratio of biologically active substances and probiotic microflora in the recipe of the complex feed concentrate is determined, ensuring the maximum reduction in the serum levels of alanine aminotransferase in the blood serum of experimental animals, which characterizes the normalization of barrier organs
131 kb

INFLUENCE OF COLOR PERCEPTION OF LIGHTING-CARE ON PRODUCTIVITY AND ENVIRONMENTALLY SAFE PRODUCTION OF POULTRY

abstract 1491905031 issue 149 pp. 0 – 0 31.05.2019 ru 35
The article discusses the impact of color lighting on changes in productivity and environmental safety of poultry products. We have given recommendations to optimize the cost of electricity for lighting the house
351 kb

PLUMAGE COLOR GENE INTERACTION IN CHICKENS (REVIEW)

abstract 1411807021 issue 141 pp. 97 – 110 28.09.2018 ru 37
Coloring the plumage of chickens is a complex genetically determined sign. The study of the interactions of the main genes of chicken pigmentation was started by A.S. Serebrovsky. The most complete information on the genes of coloring plumage of chickens and down of chickens is given by Z.M. Kogan, indicating the clutch groups and the arrangement of these genes on the chromosome map. In the 1990s, the interaction of color genes, pen pattern and modifier genes affecting the intensity of the main color and pattern of the pen was described in detail. It was found that in pigs the pigmentation is regulated by the MC1-R melanocortin receptor associated with the locus E. Due to molecular studies, loci of dominant white color (I), recessive white color (c / c), striped pen pattern (B) adherent to the floor , and spotted spot locus (mo). The final phenotype depends not only on individual genes, but also on their interactions and the genetic environment as a whole. The same genes can produce different patterns and shades of the main plumage, and different genes determine the same phenotypes. Despite the fact that there was significant progress in determining the molecular causes of the appearance of a particular type of color in chickens, much remains to be learned
14474 kb

DEVELOPING A VETERINARY TEST FOR THE DIAGNOSIS OF GASTROINTESTINAL DISEASES IN HORSES BASED ON DATA FROM THE UCI REPOSITORY WITH THE USE OF ASC-ANALYSIS

abstract 1411807033 issue 141 pp. 111 – 175 28.09.2018 ru 39
This article briefly discusses a new innovation (brought to a level that ensures its practical use) method of artificial intelligence: automated system-cognitive analysis (ASC-analysis) and its programmatic toolkit which is called intellectual system "Eidos". A detailed numerical example of the solution demonstrating the technology of creating a veterinary diagnostic test of gastrointestinal diseases of horses is given. As the source data, we use data from the UCI repository, kindly given by Mary McLeish and Matt Cecile (Department of computer science of University of Guelph, Ontario, Canada N1G 2W1, with the support of a sponsor: Will Taylor. The developed test is used to solve the problems of diagnosis, decision support and examining the simulated subject area by studying its model. The results of the study can be used by anyone, due to the fact that Eidos the universal automated system, which is a tool of ask-analysis, is in full open free access on the author's website at: http://lc.kubagro.ru/aidos/_Aidos-X.htm, and numerical examples of solving veterinary problems with the use of artificial intelligence technologies are placed as a cloud Eidos-application 129
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