A priority direction at the present stage of vegetable
growing development is to obtain high-quality,
environmentally friendly production on the basis of
development and introduction of new technologies of
cultivation. At the same time, it is compulsory to
emphasize the importance of expanding the range of
crops which can vary nutrition of population. Currently
promising direction is search of new high-efficient and
environmentally friendly methods of plant protection.
Biological methods of effect on the plant body acquire
great significance. One of such methods is using grafts.
In a light of new changed conditions, the research on
grafted plants was not conducted in our country
The article presents the materials of the study of the
features of cultivation of Lactobacillus sp. on
different culture medium. We have made a
comparison of standard culture medium for
cultivation Lactobacillus sp. and determined the
most optimal culture medium
We have presented materials about nutrient media
for growing and industrial the cultivation of
Propionibacterium shermanii: Trypticase Yeast
Extract Glucose Medium, the culture medium
Ellinger and ATCC Medium 33. We have studied pH
change and its impact on the biomass accumulation
of propionic acid microorganisms. We have also
defined the best time of cultivation
The article presents the results of three years of
researches conducted at the Department of Viticulture
of Kuban State Agrarian University, to study the
processes of shoots and rooting of the three-bud
cuttings of grapes of Pervenets Magaracha, Podarok
Magaracha and Cytrony Magaracha of the selection of
Magarach Institute of Grape and Wine. The area of
these varieties, which are inter-specific hybrids, in the
Krasnodar region is more than 2 thousands hectares.
The cuttings were rooted in sawdust in a greenhouse
on a heated rack. It was found, that the regenerative
capacity of cuttings was strongly dependent on the
method of their storage prior to installation on the
germination. The best results were obtained in the case
when cuttings had been stored in the refrigerator
before starting the experiment. The lowest impact of
the way to store cuttings was on the percentage of
cuttings with blossoming eyes, and the largest - the
length of the shoots, the output of the cuttings with at
least 3 of the roots, and the average number of roots
developed on one cutting. Rooting of cuttings and
their growth with three and more roots were different
over the years for the varieties of Pervenets
Magaracha and Cytrony Magaracha, in favor of one
sort or another, but the average 3-year difference
between varieties was not significant. On average, in
two or three years, the maximum number of roots
appeared in the variety of Cytrony Magaracha, which
was a bit ahead of Pervenets Magaracha. The
difference in the number of cuttings formed at the
roots was authentic. Correlation analysis showed that
the closest relationship to the performance of the
rooting ability of cuttings was found in the length of
the shoots. The level and the nature of the identified
correlations suggests that the rooting ability of the
cuttings of the varieties of Pervenets Magaracha was
determined primarily by hormonal activity, Podarok Magaracha by its supply of plastic substances, and
Cytrony Magaracha had equally both factors
The article presents the results of three years of studies
on regenerative processes in technical white one-bud
cuttings of resistant varieties of grapes Bianca, Viorica
and Riton. The studies were conducted in greenhouse
experiments at the Department of Viticulture of Kuban
State Agrarian University. It was found, that the
cuttings of Viorica varieties had achieved the best
ripening, and the worst was Bianca. Varieties of
Viorica and Ryton which have rather good level of
ripening of the cuttings, had starch in carbohydrates
and Bianca variety had sugar. Earlier blooming of
buds characterized the cuttings of Ryton. Blooming of
the buds of Bianca somewhat delayed. Maximum
length of the shoots characterized the cuttings of
Bianca, and the lowest – Viorica. The shortest pre-root
period (26.3 days) was observed in Ryton, and the
longest (29.3 days) – in Bianca. Viorica was
characterized by the highest yield of cuttings with 3
roots and more (81.3%), this indicator was a bit less
(72.5%) for Ryton. For Bianca it was minimal and it
was 26.3%. A similar pattern was observed for the
average number of cuttings formed at the roots: the
variety Viorica - 9.4 pc.,Ryton - 8.1 pc. and Bianca -
4.3 pc. Thus, cuttings of the varieties of Viorica and
Ryton belong to a group with a very high root growing
activity and Bianca has an average activity
The article presents the results of studies on the effect
of processing varieties of grape cuttings of Pervenets
Magaracha using alternating electromagnetic field on
their regenerative skills. Processing the cuttings with
alternating electromagnetic field was carried out using
an induction coil for 5,10,15 and 20 min. As a control
option, the cuttings were soaked in water, as a
standard option – the cuttings were soaked for 24
hours in a 0.01% solution of IAA. Rooting was
performed in vessels with water at optimum
temperature conditions. It was found, that the use of
IAA for the first time led to the inhibition of blooming
buds. EMF treatment of cuttings with each exposure
has a stimulating effect on this indicator. Application
of EMF exposures in the 5 to 15 minutes resulted in a
significant increase in the length of shoots, compared
to the control variant and the option to IAA.
Processing cuttings with EMF exposures at 5,15 and
20 minutes resulted in a significant increase in rooting
compared with the control. Maximum rooting was
obtained at an exposure of 15 minutes. It was the same
as in the version with a standard rooting stimulant -
IAA. However, the highest yield of cuttings with 3
roots and ended up in a version with a 20-minute
exposure. Maximum number was noted on the heel of
the roots cuttings option with IAA. Treatment of
cuttings with EMF exposure 5, 10 and 20 minutes, has
also led to a significant increasing of this indicator,
respectively, 45.5; 22.7 and 79.5%. The maximum
increase in the number of roots was obtained in the
variant with exposure 20 minutes. Thus, this option
should be considered as the best one
Results of testing of multiplex sets SSR-markers for
genotyping of rice varieties are presented in the article.
Two sets of SSR-markers were formed:
1: RM1+ RM11+ RM70+RM122;
2: RM164+RM167+RM168.
The optimal combination of DNA markers in the
multiplex sets and PCR conditions allowed obtaining
accurate, easily interpretable results when performing
fragment analysis on automated genetic analyzer
ABIprism3130. Using multiplex sets, genotyping was
performed for several varieties of rice: domestic
breeding and one variety – IR36 from the breeding of
IRRI (Manila, Philippines). For all the studied
varieties specific SSR-fingerprints were obtained.
RM 168 marker showed in domestic varieties a low
level of polymorphism - one allele of 97 bp. However,
at the same time, the variety IR-36, showed a second
type of allele 107 bp. In addition, the loci of RM1,
RM11, RM167 and RM164 have unique alleles in this
variety. It is consistent with significant genetic
differences of these varieties and the rest of the
varieties in studied sample. The proposed SSR
multiplexes are promising for use in DNA certification
of rice varieties and assessment of genetic diversity
In this article, we discuss the results of the research of
quality characteristics of Russian and foreign rice
varieties. Possibility of using data in characterizing
germplasm from collection is discussed
The article presents materials on studying distribution
and phytocenotic confinedness of relict steppe species
of Astragalus angarensis. Astragalus angarensis is the
endemic of the Angara region and Yakutia, it is a relict
species. Three small isolated fragments express the
area of species. The largest fragment was found in the
Angara region; the smallest two fragments are
occurred in the valley of the Lena river, in the southwest
Yakutia near Olekminsk and in the central
Yakutia, from Yakutsk to Bulgunnakhtaakh village.
Ecological and geobotanical characteristics of habit
area of Angara Astragalus is given; floristic content of
community is studied. The described desertificated
Krascheninnikovia, Psathyrostachys steppes and also
Festuca, Agropyron, Koeleria and Artemisia
communities with Astragulus angarensis confined to
slope habitats, characterized by poor floristic content
that gives evidence of low competitiveness of this
species
Novoafonskaya cave is located in Abkhazia. It is
equipped for visits in 1975. The cave has permanently
installed lighting. In caves with artificial lighting, a
vegetation of cyanobacteria and algae, bryophytes and
ferns can be found around lamps. The development of
lampenflora is a typical problem for cave management.
We have identified 69 species of phototrophs in
Novoafonskaya cave: Magnoliophyta 2 species,
Pteridophyta 6 species, Bryophyta 11 species,
Cyanobacteria 34 species, Bacillariophyta 9 species,
Ochrophyta 2 species, Chlorophyta 5 species. The
article considers main habitat of lampenflora and gives
their characteristics. We have also revealed
predominance of cyanobacteria in the cave