In the article we have stated results of the monitoring of local populations of rare plans species in the territory of the nature monument (NM) of the Rostov region «Raznotravno-tipchakovo-kovylnaya steppe» (Zernogradsky district). Vegetation of NM belongs to the poor version of the Azov steppe, flora contains 291 species, including 13 rare species (Astragalus ponticus Pall., Bellevalia sarmatica (Pall. ex Georgi) Woronow, Calophaca wolgarica (L. f.) DC., Caragana scythica (Kom.) Pojark., Centaurea talievii Kleop., Crambe pinnatifida R. Br., C. tataria Sebeok, Crocus reticulatus Stev. ex Adam, Eriosуnaphe longifolia (Fisch. ex Spreng.) DC., Iris pumila L., Stipa pulcherrima К. Koch, S. ucrainica P. Smirn., Tulipa schrenkii Regel), included in the Red List of the Rostov region, including 6 species included in the Red List of the Russian Federation. In 2016 were specified the patterns of rare species distribution in NM borders, and also other important parameters of their populations (number, density, age structure, etc.). The group of rare plants species, included in the Red List of the Rostov region, in the territory of NM includes 65 % of their total number known in Zernogradsky district. The special sozological value of NM is determined by the large local populations of Calophaca wolgarica, Astragalus ponticus and Crambe pinnatifida. Local populations of all rare species in the territory of NM can be considered to be steady as inhabit here during last 50 years
The results of introduction test of species and cultivars
variety of irises in the Central Yakutia are described in
this article. Climatic conditions of the introduction
region are extreme and they are strong restrictive
factor at introduction to the culture of many other
species and cultivar of decorative plants. The condition
of plants after rewintering was estimated on a 4-mark
scale. Total number of the plants which were lost in
the first winter (0 points) is 126 species and cultivars
that is 60,9%. For 01.06.2015 year a collection of
irises in Yakutia makes 40 exemplar of species (22
species) and 31 exemplar of cultivars (30 cultivars), 7
species of them are successfully adapted (3 points)
(Iris biglumis Vahl., I. bloudowii Ledeb., I. potaninii
Maxim., I. scariosa Willd., I. setosa Pallas ex Link.,
I. tigridia Bunge, Pardanthopsis dichotoma (Pallas)
Lenz). The listed species annually plentifully blossom,
form seeds and intensive form escapes. Four species
Iris biglumis, I. scariosa, I. bloudowii, Pardanthopsis
dichotoma are form stable self-seedling
The problem of increasing resistance of winter
barley to adverse conditions of winter is the most
problem in the selection area of this crop. The main
component of this complex trait is the frost
resistance. Laboratory methods of assessment of
frost resistance are important for breeding of winter
barley. These methods can effectively replace more
costly method of direct freezing in the freezers.
Magnesium cations (Mg++) are essential
components of the protein-synthesizing system of
germinating seeds. Magnesium cations determine
the stability and translational activity of mRNA and
rRNA. Trilon B is usually used for in vitro banding
of magnesium cations in area of biochemistry of the
nucleic acids. It was very interesting to verify the
Trilon B influence to seed germination of winter
barley varieties by experiment. Experiments
showed: actinomycin D – the inhibitor of RNA
synthesis – didn’t show varieties specific effect to
seed growth of winter barley, while Trilon B had a
varieties specific impact to length coleoptiles and
roots. Research was carried out on etiolated
seedlings at temperatures from 22 to 28oC, and different concentrations of Trilon B - 1,6×10-3М,
2,4×10-3М and 3,2×10-3М. The roots of 3-days old
seedlings were more sensitive to Trilon B influence.
It is shown the varieties of Russian origin were
naturally reacted to Trilon B: the higher the frost
resistance of variety, the more resistant variety to
the action of various concentrations of Trilon B at
different temperatures. The most optimum
concentration was 3,2 × 10-3М at 22-25oC.
Varieties by foreign origin deviated from this
pattern. It is interesting to research the genetic
essence of these differences. It will be helpful for
optimization of varieties differentiation conditions
to create a laboratory method of estimate of frost
resistance of winter barley. The new method will be
most efficient compared the direct freezing method
Along with traditional ways, the biotechnological
methods become more significant in contribution to
plant conservation in situ. The work is dedicated to
elaboration and advancement of the method of isolated
tissues and organs culture for conservation and
reproduction of the badan, Bergenia crassifolia L., the
rare species of Saxifrgaceae family. The conducted
study yielded the regenerated plants of B. crassifolia
from seeds. For the first time, we have performed
conservation and reproduction of B. crassifolia in vitro
under conditions of Yakutia
The article presents monitoring of the course and the
beginning of new invasions due to the intensification
of these processes in the basin of the Azov Sea of;
investigation of the influence of the alien species
upon the acceptor ecosystem. Methods. The material
was collected at the monitoring transect in the Don
River delta and in the surveys of the r/v “Professor
Panov” in the Taganrog Bay, Sea of Azov. Standard
hydrobiological methods of the data collection were
used. Results. Penetration of three polychaete species
was recorded; course of invasion of two of them was
traced. Impact upon the acceptor ecosystem was
researched. Preliminary species identifications using
morphological characters were carried out; sources
and the ways of the invasions are suggested.
Conclusions. Estuary zone of the Sea of Azov (the
Taganrog Bay) has a high invasion capacity. Of the
three species penetrated into the Sea of Azov basin in
2013-2015, one (Aracia sp.) naturalized successfully,
though didn’t affect much the ecosystem. On the
contrary, polychaetes of the genus Marenzelleria are
increasing rapidly their area; in two years, they
became dominant along the considerable part of the
water body. The invasion of Streblospio sp. is at its
very beginning at the time
In FSBRI «North-Caucasus zonal scientific-research
institute of horticulture and viticulture» (Krasnodar)
there was carried out the comparative economicbiological
study of the average-height apple-tree
stock М9ЕМLA in comparison with initial weakgrowing
stock М9 in manifold, nursery and garden.
Manifold bushes of the clone M9ЕMLA is quite
worse in force than the stock М9 and were less
branchy. Output of standard per hectare of manifold
at the clone M9ЕMLA was on 35,5 thousand units
more, and а standard condition of cuttings - on 13,2
% higher, than at the stock М9. Cuttings of the clone
were less spiked and better rooted. In the first field of
nursery, the plants of the clone had a vertical growth
of the central conductor, slightly weaved and were
more suitable to inoculation. The output of standard
plants, in the variant with the use of clone M9ЕMLA
by the variety Champion, in average for 2006-2010
was on 3112 units/hа, and on the variety Prikubanskoye
– correspondently on 2470 units/hа higher
than grafted on the stock М9. The cost price of 1000
seedlings on the clone M9ЕMLA was on 1962 rubles
less than on the stock М9, and the standard of profitability
of seedling growing, on the contrary was on
14-19 % higher. In the garden the trees on the clone
M9ЕMLA were worse in growth of trees grafted on
М9: on volume of the crone on 0,4-0,6 m3
, in diameter
of the stem on 9-12 mm, in dependence on
the variety, independently on the fact that some trees
on clone were more weaker than grafted on the stock
М9, their productivity for 2011-2015 was higher as
from one tree as from the unit of area. The intensity
of fruiting expressed in yield in kilos from a tree on
the unit of volume of the top of a tree on the clone
M9ЕMLA was on 33-39% higher than at the trees on
the stock М9
The phenomenon of fasciation on the example of a higher plant called “Taraxacum officinale Wigg” has been considered. The effect of fasciation on the various particularities of plants has been shown. There are numerous examples of the different fasciation types of present plant’s type which are growing in different ecological areas. We have demonstrated that the phenomenon of fasciation exists in natural populations and in areas with development pressures, and it may occur after mechanical damage to plants of Taraxacum officinale. In most cases, fasciation is regarded as an exceptional phenomenon. A number of authors have distinguished fasciation to non-inheritable and inheritable. The first is the influence of external factors such as insect damage, injury, weather conditions. The second is caused by internal reasons. The number of regularities was installed that the reproductive bodies of plants are more often fascinated, and thermophilic forms are more likely to have fasciation. Photographic images of various types of fasciation, patterns and diagrams are given. Fasciation can be considered as a marker because of the connection of this phenomenon with the violation of the ecological situation. Plants with morphoses including ones with fasciation of individual organs, can act as indicators of pollution of the natural environment. Thus, the phenomenon of fasciation affects many areas of science, such as ecology, morphogenesis, genetic monitoring. The possibility of studying the phenomenon of fasciation on a model object of dandelion is shown. As a methodical approach to the study of this phenomenon, it is proposed to create a database of images, in this way it would be cataloging the image
Two granular formulations of phosphorus biofertilizers
combining rock phosphate and two highly active
phosphate solubilizing strains: Acinetobacter species
305 and Pseudomonas species 181а have been investigated.
Granules of about 3 mm in size were obtained
by contact-convective drying of a mixture of ground
ore, concentrated biomass of two different strains,
starch and glucose. Micro granules with size of 0.1-
0.5 mm were obtained by spray drying the biomass of
two different strains and application of dried cells on the particles of the ground ore. Starch was used as a
binder. In the model liquid medium it was shown that
the microorganisms have retained the ability to solubilize
mineral phosphates in granular formulations prepared.
In laboratory pot trial on marigold (Tagetes
patula) it was demonstrated that both formulations of
biofertilizer increased the dry weight of the plants to
the same level as that of chemical fertilizer - double
superphosphate, but were inferior in the concentration
of phosphorus in plants. Both formulations exceeded
the effectiveness of rock phosphate and biomass used
as biofertilizers both separately and jointly. No significant
differences were noted between the two
strains and the two granular formulations both for
plant dry weight, and the content of phosphorus
therein. Both granular formulations of biofertilizer
retained their structure and avoided aggregating over a
year of storage at 4 oC. The average persistence of
living cells in the microbeads was about 1.5%, in
granules - 32 %
The purpose of examining the roadside agrocenosis in the Aksai district of the Rostov region was a comparative assessment of the ecological condition of ordinary black soil and agricultural products grown in the technologically transformed conditions. The evaluation of agro-forestry factor impact on refraining toxic substances from the techno-genesis road source was also of interest. The study was expected to determine the actual and potential content of copper, zinc and lead in the ordinary black earth, as well as to evaluate the accumulation level of the investigated elements in the winter wheat grain. The potential reserve of investigated elements in ordinary chernozem (or black soil) is several times higher than the potency of the current content. Distribution of the results of the actual and potential reserve determination demonstrated the transfer of gas-dust emissions beyond the forest belt where the production crops are grown. At the remoteness point of 10 meters away from the road beyond the forest belt minimal amounts of actual and potential element content were found. At a distance of 50 - 100 m there was found a significant excess content of copper, zinc and lead. Repeated studies conducted 20 years later, identified the lead content in winter wheat grain exceeding the MPC. The results obtained have demonstrated the lack of agro-forestry effect in refraining gas and dust emissions by the road forest belt
The article deals with the topical issues of ornamental
woody plants used in street landscaping of Sochi
Central district. It presents the quantitative
composition of these species, numbering 12181
specimens. With the scale, modified for the regional
conditions, we carried out an analysis of the studied
plantations and their state, which revealed that
weakened plants were predominant - 72.48%. Healthy
plants make up over 20%. The lowest number of the
specimen was recorded among the dead plants
(0,26%). The analysis was carried out for all species
according to the state categories. For instance,
structure-forming species selected for further studies,
were analyzed this way, including a large part of plants
that belong to the second category of state (89,04%).
Healthy plants are less than 8% from the total number
of dominant species. The number of dead plants makes
up 0,35%