The article gives the results of the study of an effect of
mechanochemical powder of wood green of Abies
(MPA) on potato morphogenesis in vitro. The study
involved the hormone-free nutrient mediums (MS, ½
B5, ½ LS) for growing microcuttings from preliminary
obtained aseptic plants. Plant morphogenesis was
observed on the 15th and 30th days. At that, the number
of developed shoots, plant height, leaves number,
rhizogenesis rates, root number and length were
estimated. MPA appeared to have the regulatory effect
on morphogenesis of potato microcuttings. Based on mineral composition of the nutrient agar medium for
potato microcuttings growing, MPA at 100 mg/l
concentration induced root development (½ B5
medium), reduced height of shoots (MS medium),
while the number of leaf blades was not affected
In root exudates of alfalfa, wheat and radish we have
found 12 amino acids. As well as 5 organic acids
were identified. In the variant with exudates of wheat
as the sole source of carbon and energy maximum
titer of microorganism Rhodococcus erythropolis
RCM Ac-2017D were observed
The article deals with hawthorn Dagestan. It is noted
that the more than 100 species are found 14 species in
Dagestan. The most common of them are studied as a
source of biologically active substances and nutrients,
and they can be used as food additives for various
purposes. The content of biologically active substances
and nutrients are defined. The high content of phenolic
substances in C. pentagyna Waldst. et Kit, which play
an important role in the formation of immunity and
possessing P-vitamin activity is defined. Amino acid
and fatty acid composition of the fruit of the hawthorn
Crataegus pentagyna Waldst. et Kit, as the most
valuable for using as food additives is defined by ionexchange
and gas-liquid chromatography. Sixteen
amino acids were identified and quantified in the
protein component of hawthorn fruit. The high content
of essential amino acids such as lysine, threonine,
valine, methionine, leucine, isoleucine, phenylalanine.
The amount of essential amino acids is 296.1 mg / 100
g. Chromatographic separation of fatty acid methyl
esters has enabled us to establish the presence of the
following fatty acids: palmitic, oleic, stearic, linoleic
and linolenic, including established the presence of
essential fatty acids - the family of alpha-linolenic acid
OMEGA -3 and linoleic acid family of OMEGA -6
At present, the demand of producers and the market
determine the model of future varieties. Task for
breeders is to develop varieties having a set of
economic and biological traits fitting into the model
templates. The developed varieties and hybrids should
ensure for climatic conditions of the Krasnodar region
consistently high yields, better product quality and
maximum efficiency of crop industry. As a result of
breeding work there was developed a watermelon
variety called Yubilyar, belonging to medium-ripening
group, having high figures of economically valuable
traits. The variety has resistance to unfavorable factors
of growing conditions and stable yields. It is suitable
for cultivation in rainfed areas and practically does not
require protective measures against major diseases
prevalent in the southern regions. Fruits are large, of
wide elliptical shape, with bright crispy red flesh and a
thin crust. Determined by stable fruiting under
different weather conditions, friendly yield return,
commercial fruits – up to 92%. Potential yield – up to
25 t/ha. Good stored for 40-50 days, suitable for
transportation on a long distance. Passed preliminary
tests on the fields in the Krasnodar, Rostov regions and
the Republic of North Ossetia. According to results of
evaluation from producers the variety meets
requirements of the market and has a commercial
appeal
The new feed additives for animals based on milk
whey, enriched with sprouting wheat, barley, maize
grains and lactic-acid bacterium have been presented
in this article. This study explores the possibility of
combining the prebiotics ability of milk whey and
sprouting grains with feed probiotic attributes of microbe
to enhance gut health of animals and digestibility
of the feed. Twelve variants of products were tested
in the study using the microbial and physical-chemical
approaches. All the assays showed high count of microorganism
and high content of reducing sugar. The
results of investigation indicate that selected feed additive
show high quality. An additional point is that it
contains useful organic acids (lactic, acetic and propionic
acids) and 2,1×109
colony-forming unit of probiotics
microorganism that hold the concentration for 4
months. There are not yeast, must, coliform bacteria
and Staphylococcus aureus in the developed functional
feed product. It contributes to the normalization of the
microflora of the gastrointestinal tract of animal, suppression
of conditionally pathogenic and putrefactive
microflora. The elaborated feed component will help
to provide combined feed companies with accessible
high-quality raw material
This work considers the needs and methods of phage
therapy of two bacterial infections in poultry. The
review examines the use of bacteriophages, from the
moment of their discovery to the present day. We
discuss the relevance of such diseases as
compilobacteriosis and colibacteriosis transmitted
from birds to humans, we show how to use
bacteriophages and their efficacy in models of adult
birds and chickens, including information provided on
the pilot study of phage therapy at a poultry farm. The
reported study was partially supported by RFBR,
research projects No. 16-44-230855 and No. 13-04-
00991 and № 16-44-230855-р_а
The soil by it physical and chemical properties is a
poly-dispersed heterogeneous multicomponent unique
environment for development of most microorganisms.
The soil is the richest natural substrate according
to microbial gene fund. Presence of plants and
animals in soil maintains it heterogeneous as environment
of soil microorganisms, which are main regulators
of natural gas composition of atmosphere of
Earth, including its macro- and micro-components
(including the main "greenhouse" gases - methane,
carbon dioxide, nitrous oxide). Recently, the elucidation
of these singularities of vital activity of soil organisms
has resulted in general conclusion - due to
them soil cover serves as a global bio-geo-chemical
membrane, through which the exchange of matter and
energy between pedosphere, lithosphere, atmosphere,
hydrosphere and main living inhabitants of Earth does
its work
The article presents materials about selection and
optimization of the nutrient medium for cultivation
of Propionibacterium shermanii. We have shown a
high positive effect expressed in the intensive
growth of microorganisms of Propionibacterium
shermanii on optimized medium with the juice
from the tomatoes, as well as replacement of
glucose on corn extract, which allows concluding
that these components have a positive impact on
the accumulation of biomass of Propionibacterium
shermanii
Microsatellite DNA markers are currently used
effectively in the study of the genetic diversity of the
gene pool of fruit crops and DNA certification of
varieties. For plum now there is rather limited list of
works on the development of this type of DNA
markers. Most often, the search for new SSR-markers
for this species is carried out by checking of crossreproducibility
of SSR-markers developed in other
species of the genus Prunus. In this study, for the 18
SSR-markers previously developed on a peach, there
was performed testing and evaluation of the prospects
for the use of the genotyping of plum cultivars. Testing
was made on the 4 varieties of genetically distant,
belonging to the 4 different subspecies of Prunus
domestica L., showed the effectiveness of their use.
During the study, all tested DNA-markers were
grouped together in multiplex sets comprising 3-4
markers. This allows simultaneous genotyping of 3-4
loci in a single PCR reaction. These multiplex kits are
available for use in the study of genetic polymorphism
of species Prunus domestica L
In the article we consider illustrative images of rare
pumpkin cultures presented in stamps of different
countries. In this work, we analyze stamps which
represent rare pumpkin cultures. Stamps are reflection
of the economy; they portray agriculture and elements
of technology of agricultural production.
Cucurbitaceae family includes a large number of
species that are very different from each other in the
form of fruits, in purpose, in origin. The objective of
our study was to conduct a visual analysis of rare
pumpkin crops, which are used in the world; to reflect
the research progress on the introduction and the
achievement of breeding work. The article provides an
analysis of several cultures: momordika (Momordica
charantia L.), Kiva (Kiwano), horned melon, cucumber
Antilles, (Cucumis metuliferus L.); Akantositsios
Naudin (Acanthosicyos naudinianus L.); momordica
balsamina (Momordica Balsamina L.); koktsiniya
(Coccinia sessifolia L.); luffa cylindrical (Luffa
cylindrica L.); Lagenaria (Lagenaria siceraria
(Molina) Standl.); cucumber snake (Trichosanthes
anguina L.); antilles cucumber, anguria (Cucumis
anguria L.); african cucumber (Cucumis africanus
Lindl); wax gourd (Benincasa hispida (Thunb)
Cogn..); chayota or mexican cucumber (Sechium edule
Swartz). The analysis of the iconography of images of
rare pumpkin crops in stamps of different countries
allowed us to see the history of culture introduction of
wild pumpkin from the local and foreign flora