Nutrient media for shoot development from shoot tips 0.5-0.8 mm with several leaf primordia in four grapevine genotypes were optimized by means of a mathematical design of experiment. Over the range of component concentrations CaCl2 had considerable the highest effect on shoot tip development compared to other components of the media. Grapevine genotypes were different in their needs for concentrations of macro-elements to optimize the process of their growth and development. Shoot development from shoot tips in each grapevine genotype as a function of macro-element concentrations in media must be described by an individual regression equation. The proposed method of result evaluation and the mathematical design of experiment may be used in physiological and agricultural research for optimization of processes affected by numerous factors. The optimized media for shoot tip development may by used for sanitation of grape plants from viruses in meristem cultures
Two methods were developed, one to assess experimental results by reducing a number of developmental parameters to the overall quality, leading to the overall quality criterion, and the other to optimize processes affected by numerous interacting factors, in-vitro plant development in this case, by applying a mathematical design of experiment. Single-bud cuttings with one leaf of two Vitis vinifera L. genotypes were excised from the central part of two-month-old in-vitro grown plants and used as explants. The explants were established on bridges of filtering paper in liquid media and on solid media. Eighteen modifications of these media contained five macro-elements, each at three concentrations, and the distribution of these macro-element concentrations followed the law of random numbers (Experiment I). Parameters characterizing arm, leaf and root development of two-month-old plants of each study genotype established on each liquid or solid medium were reduced to the overall quality of plant development. Since the study genotypes differed in the ability to grow on liquid and solid media with different macro-element concentrations and also in the ability to utilize these macro-elements, the dependence of in-vitro plant development on macro-element concentrations was described by different regression equations. That is why the regression equation describing the average outcome of plant development in the two genotypes on liquid and solid media was not as significant and the description of the process was not as adequate (determined) as regression equations calculated for each process. The regression equation which describes the dependence of the average outcome of plant development on macro-element concentrations in Experiment I is as follows: y5 = 0.027 + 0.116x22 + 0.109x2x4 + 0.106x2x3 + 0.114x4. Stepwise calculation of macro-element concentrations to optimize in-vitro plant development was done based on macro-element concentrations of the initial medium of Experiment I as starting points since that medium was best efficient for this purpose by using regression equation (y5) and algorithms of multiple curvilinear stepwise regression according to the Box-Wilson method of steepest ascent. Experiment II was undertaken where macro-element concentrations (‘steps’) were calculated in a stepwise manner to optimize in-vitro plant development of the two V. vinifera genotypes and the rootstock ‘Kober 5BB’. This led to a liquid medium and a solid one which enabled a better plant development in the three genotypes relative to the use of controls: media with ½ MS macro-elements and the initial medium whose macro-element concentrations entered as starting points to calculate ‘steps’ for optimization of in-vitro plant development. The optimized medium contained macro-elements: 318 mg l-1 NH4NO3 (x1), 1188 mg l-1 KNO3 (x2), 370 mg l-1 MgSO4 7H20 (x3) (MS), 370 mg l-1 KH2PO4 (x4), 331 mg l-1 CaCl2 (x5) (MS), and other substances at optimum concentrations adjusted earlier: ¼ MS Fe-EDTA, ¼ MS micro-elements, 20 mg l-1 myo-inositol, 0.1 mg l-1 thiamine (MS), 0.5 mg l-1 nicotinic acid (MS), 0.2 mg l-1 pyridoxine, 2 mg l-1 glycine (MS), 0.1 mg l-1 indole-3-acetic acid, 10 g l-1 sucrose and, only for solid media, 7 g l-1 Difco agar. The optimized medium may be used for propagation of virus-free plants, valuable clones and grapevine genotypes created by gene engineering. The mathematical design of experiment reported in this paper which enables stepwise optimization of in-vitro plant development may be used both in agriculture and in the food industry
This article describes the developed algorithm for optimal load distribution between heterogeneous systems in terms of performance when working with a distributed web application
The article has examined the activities of subjects of the innovation infrastructure in the agriculture of the Chuvash Republic, offered to create the Chuvash cen-ter of agricultural innovation transfer of technologies, whose activities will include all phases of the devel-opment to innovation
The article highlights the questions of preliminary determination of the yield of vineyards, grape harvest prediction method to determine the optimal length of fruit cutting of shoots and load bushes wintering eyes, kidneys examined productivity index wintering buds (CP), which shows how many inflorescences have an average for each left eye escape when cutting to length, including the dead. It was found that the yield of grapes has a great influence the degree of differentiation of inflorescence of rudiments in the central buds overwintering buds. From the moment of laying the harvest of grapes in hibernating little eyes in the form of embryonic inflorescences until commercial harvest takes two growing seasons. During this period the generative organs of grape plants are affected by many factors of the environment. Heterogeneity was found of buds along the length of one-year matured shoots. It is expressed in the fact that many grape varieties fruitfulness buds at the base of the shoot are much lower compared with upstream eyes. A certain death of wintering buds (primarily, the main kidney) at the end of the growing season, before the autumn frosts. The main reason for the death of buds during the growing season - from defeat fungal diseases. Taking into account the percentage of deaths of buds proposed a new exponent forming embryonic kidney main inflorescence - productivity index of buds by the ratio of embryonic stems to the number of buds, including the dead. Thus, annual forecasting of grape harvest next year to cutting the bushes by the proposed method makes it possible to scientifically establish the optimal cut length of shoots and fruit load on the bush eyes. This helps to realize directly the potential of each variety and vineyard site and get maximum yields even in years with low fruit tab formations
The article provides an optimum flow of the adhesive used in laminating of shield parts by sliced veneer while padding. The influence of the wetting ability of adhesive composition on the strength of veneer bonding to the base and allowable storage time of veneer treated by adhesive composition are examined
The food supply has a great influence on the development of animal husbandry. It is its basis. Health of animals, quality and quantity of the made production are dependent not only on the amount of the prepared forages, but also to a greater extent on their quality and balance. The quality of the prepared forages has an impact on their consumption and digestibility. Therefore, the more qualitative the forages are, the higher the level of the productivity of animals is. High level of the organization of the balanced diets reduces costs of forages per unit of production by 1,5-2 times in comparison with unbalanced diets. This fact once again proves the importance and necessity of biologically full-fledged diet of feeding animals. The full-fledged diet should contain the optimal quantity of digestible protein, fodder units and other nutrients. Indeed, owing to full feeding, the productivity of animals increases and the body weight increases as well that, in turn, has a positive impact on the economic condition of the subject. However, rational use of forages requires determination of their quality and energetic and protein nutritiousness. To produce optimum diets in which all above-named criteria are considered, special programs for optimization of diets can be used that allow to provide animals with forages containing all the necessary nutrients at minimum expenses. These programs are based on the methods of linear programming, which lie in finding optimum plans for the problems with linear structure. For the solution of linear programming problems the simplex method is usually used which in a general view means that with the help of a complex of consecutive procedures the solution of a problem that meets an optimality condition is found. In this article, the problem of minimizing the cost of a diet for bulls will be solved with the use of the program named Optim V 2.0
The optimal angles of spatial orientation of disk tools are found basing on the mathematical model of the universal soil-processing instrument. The optimization problem has solved basing on analytical approximation of imitational modeling results and optimization map analysis
The article is a study of digital continuous system and
optimization of digital control systems. It is shown that
the standard in the synthesis of digital systems should
provide a continuous system, so first we need to create
the desired continuous system and then we could try to
get the digital system closer to it
The article presents growth rates, formation of the fruit of the entities and the fruits of apple varieties of “Florina”, “Zolotoy potok” depending on the strength of growth stocks and planting schemes