Studying natural phenomena in all their diversity,
humanity worked experienced in every field of
science the model of perceiving the world and
methods of obtaining information. The development
of science currently cannot be imagined without
research on the intersection of its regions. This
article presents the results of the automated systemcognitive
analysis of the size of atoms from the
main characteristics that are of research at the
interface of General chemistry elements and
intelligent systems. Dependence of nuclear radius,
mass and of the atom and the charge number are
identical in shape and size, which is probably
connected with the linear increase of these
parameters in the Periodic system of chemical
elements. There is also a similar form of the
dependences of radii of atoms from the factors ex
and x, because these factors are interrelated. The
obtained results of the ask analysis have confirmed
the theoretical assumptions and the formulae of the
dependence of main characteristics of the atom
The new benzimidazole-functionalized graphene
material based on graphene oxide (GO) and 3,3',4,4'-
tetraaminodiphenyl oxide (TADPO) was obtained
under one-step hydrothermal synthesis conditions.
According to IR spectroscopy and elemental analysis,
as a result of the reaction, benzimidazole (BI) rings are
formed, and the mass content of nitrogen is 12.3%.
The new graphene material is characterized by
excellent electrochemical efficiency in a threeelectrode
supercapacitor. As a result of the redox
activity of BI cycles the specific capacitance reaches
286 F/g at the scan rate of 2 mV/s, which is
substantially higher than the reduced graphene oxide
(RGO) sample obtained under similar conditions
without using TADPO (159 F/g at 2 mV/s)
The change in the Kepler’s atomic number in the
Periodic Table of Mendeleev is studied. It is shown
that its values decrease regularly with increasing
nuclear charge. An alternative formula for computing
the values of the Kepler’s atomic number is proposed.
It is shown that the ratio of the atomic number of
Kepler’s to the mass of the atom is a constant for each
period. The values of these constants are calculated
The article presents the structural and phylogenetic
analysis of VIT_02s0033g00410,
VIT_02s0033g00390 and VIT_02s0033g00450
genes of the grapevine genome and the closely
related orthologous gene MYB114 of the
Arabidopsis genome. These genes are responsible
for the biosynthesis of anthocyanin in the organs of
model plants and are of interest not only for practical
production and breeding, but also for fundamental
research. These genes were analyzed for GCcomposition
of nucleotides, the presence of cisregulatory
elements and promoter regions. DNA and
protein sequences were aligned to look for similar
elements, which allowed further analysis of the
ultraconservative domains of four genes. Based on
the results of search and identification of the
conservative regions, a cluster tree was constructed,
which made it possible to identify the separation of
gene sidelines from, presumably, the main one. At
the same time, the construction of consensus trees
based on DNA and protein sequences revealed their
absolute similarity. "The Minimal Evolution Tree"
allowed calculating the approximate dates of the
appearance of the mutations and the divergence
times of the gene branches between each other. At
the same time, the appearance of the Vitis genus and
its separation from the Rosales was taken as the time
first divergence point. In the end, homologous
metabolic pathways were searched between
grapevine and Arabidopsis, which revealed the
presence of homologous proteins in the grape
proteome. In this turn, it already confirms the
existence of similar biosynthetic pathways and, as a
consequence, interactions such as "DNA-protein"
and "protein-protein"
A method of obtaining insertion mutants for the hoc
gene, which encodes for the main phage antigen, was
developed on the model of bacteriophage T4. This gene
was cloned in the plasmid pBSL0+ and was disrupted
by insertion of foreign DNA. The phage mutants were
obtained by in vivo phage-plasmid recombination. The
construction of insertion bacteriophage mutants was
carried out in two stages. The resulting mutants on this
procedure could be grown on wild-type E. coli strains,
which is convenient for the production and use of these
phages in therapy. The mutants obtained had reduced
antigenicity. At the same time, the yield of the mutant
strains was high when they were grown on the
non-suppressor E. coli laboratory strains. A number of
stages of purification of the bacteriophage mutants
obtained were performed. Preparations have been
studied by transmission electron microscopy and mass
spectrometry. By several periodic cultivations of the
mutant bacteriophages, it was shown that mutations of
this type are stably maintained during more than 50
generations. T4 related bacteriophages of the family
Myoviridae, for example, T-even, have the significant
homology amongst their genomes, which makes
possible to produce similar mutants. Thus, our method
was developed to obtain mutants with reduced
antigenicity which can be used for both the treatment of
systemic infections, and diarrhea in the case, when,
bacteriophages penetrate into the bloodstream. Such
phages can be used in medicine and veterinary. The
reported study was partially supported by RFBR,
research projects No. 13-04-00991, 16-44-230855
The analysis of the accumulation and distribution of
heavy metals in the stevia raw material grown in
different agroecological regions is carried out. The
elements present in the soils always have a complex
effect on the plant and at the same time enhance or
weaken each other's action. Plants of stevia are able to
regulate the flow of the heavy metals under study,
assimilating organs accumulate the optimal number of
elements necessary for their growth and development,
regardless of the growing conditions
This article presents the results of testing IRAP DNA
markers Cass1 and Cass2 applied to Prunus spinosa.
The findings suggest the high perspectiveness of
their using for the study of genetic diversity of the
gene pool of this species. According to the results of
the analysis of the sample 12 genotypes were
identified from 6 to 13 fragments in the spectrum of
Cass1 and from 5 to 11 fragments for Cass2. As a
result of cluster analysis in the sample formed three
groups of samples. In one of the groups, which is
most distant from the other two, includes samples
taken in Ukraine, while the remaining two groups
included samples from Armenia, the Krasnodar
region region, the Republic of Adygea, Ukraine and
Moldova, and three cultural large-fruited form. The
distribution of samples in clusters corresponded to
their geographical origin that favors the objective
assessment of genetic distances between the samples
using Cass1 and Cass2 markers. Thus, it was
concluded that the prospects of using DNA markers
to study the genetic diversity within a species of
Prunus spinosa
Species composition and abundance of soil algal flora
were studied in the Central zone of the Krasnodar region
on crops of crops cultivated by various technologies. In
the algal flora of the studied area, 48 species of soil algae
and cyanobacteria from 28 genera and 18 families, 7
classes and 4 divisions were identified. The largest and
numerous in number of species are the departments:
Cyanophyta and Chlorophyta. Extensive delivery:
Oscillatoria, Gloeocapsa. They account for 22 % of the
total number of species. A negative effect of herbicides on
number and species composition of soil algae was noted.
In all variants of their application there was no form of
Klebsormidium flaccidum (Kützing). The number of
species of Chlorella vulgaris Beyer. was low,
Botrydiopsis arhiza Borzi. It is believed that these species
of algae can be used as bioindicators. The positive effect
of mineral fertilizers and the aftereffect of introducing
organic matter on the soil algal flora have been
established. In these variants the deleterious effect of
herbicides was reduced. According to the calculated
regression equations, the applied mineral fertilizers to a
greater extent weakened the negative effect of herbicides
on the total number of populations than the aftereffect of
introduced organics
The article describes the synthesis and verification of
statistical and system-cognitive models of the
influence of environmental factors on the quality of
life of the population of the region. This stage of the
ASC-analysis is performed in the system called
"Eidos". As a result, we have created and validated
(verification stage) all the specified systemic cognitive
models. It is expected that reliability for the models of
knowledge is sufficiently high for a given subject area,
that is why we can state the discovery of a dependence
of life expectancy and causes of death from
environmental conditions. Typically, knowledge
models are approximately 20% higher in accuracy than
statistical models, which operate on the principle of
positive pseudo-prediction. Making decisions based on
the model of Abs (matrix of absolute frequencies) is
not appropriate because of the different number of
instances of classes (generalized categories) and
dependence of the solutions of this amount. In the
model called Prc2 (conditional and unconditional
percentage distribution) the dependence of the model
values of the number of examples in classes has been
removed, but the accuracy of it is usually same low as
in the Abs. In addition, for decision-making based on
this model, one has to compare the values of
conditional and unconditional probabilities manually,
which is laborious and hardly possible for large
dimensional models. The knowledge model called
Inf3, based on a measure similar to the Chi-square, is
the result of the automated comparison of values of
conditional and unconditional probabilities presented
in the model of Prc1, which is similar to Prc2, and
usually has a fairly high accuracy, especially
considering the high complexity of the subject area,
which we simulated. Therefore, in accordance with the
technology of the ASC-analysis data conversion into
information, and afterwards - into knowledge, it is the
model of Inf3 which is planned to be used for the
solution of problems of identification, forecasting, decision-making and exploring the modeled subject
area, through the study of its models
The aim of the study is to investigate the
regularities of deflection in the diagonal and radial
tires for the grain combine propulsions of high
productivity. The research method is experimental.
The "tire tester" and specially designed devices for
revealing the deflections of the inner and outer tube
relative to the rim in the circumferential and radial
directions have been used. The outcome indices of
the pneumatic-tired wheel are determined with the
tire capability to deflect so, that at low - hysteresis
losses in the rubber-cord casing and its acceptable
tensions, based on a resource, the largest area of
contact with supporting base would be created. This
circumstance acquires the particular significance
developing tires for grain combines of high
performance, the deflection regularities of which
are poorly studied at present. The analysis of
experimental studies showed the feasibility of using
the grain combine propulsions instead of the
diagonal radial tires of the same size. The resistance
to rolling of radial tires 30.5 R-32 is lower than in
diagonal tires 30.5 L-32, and the developed contact
area due to the increase of length is higher, which
reduces sealing effect on the soil