Scientific Journal of KubSAU

The clone selection has very outstanding position behind the actual problems of modern viticulture in the world and in our country. This problem usually has the primary importance behind the scientist who works with grape in all the countries such as USA, German, French, Spain, Italy, Hungary, Bulgaria, Moldavia, Ukraine and etc. In our country last years some positions becomes solving in the plane of scientific research work. Consequently, in the article the history and the situation of clone selection of grape on the Eurasian continent is widely covered.

The global warming of the planet caused big changes of biological and social-economic character on the continents. It leads to necessity to invent new varieties of grapes, which are having higher adaptation to changes of environment, including practical steadiness for higher summer temperature and drought. The genetic improvement of Greece varieties was made by the methods of combine selection. The variety Likovrisi was made in Athens University of grape by P.Zamanidi and L.Troshin in 2001 by crossing of varieties Savvatiano and Ugni white. It has higher yielding. The productive period is middle-late. The bunch is big, wings, friable. The berry is middle, round, green-yellow. The pulp is juicy, taste is harmonious. The sugar is higher. It has higher warm-drought steadiness and higher stability to mushrooms diseases in compare with it’s parents. The variety is used for making dry white wines of different categories and spirits for cognac, brandy and grape vodka

Characteristic of chemical composition of varieties of grape cultivated in the Central zone of Krasnodar region and quality of juice produced from them is given in the article. Varieties for manufacturing of high-quality juices are recommended

Effects of different levels of vitamins (thiamine, pyridoxine and nicotinic acid) and macro-elements (MS, NN and PG) on development of globular, heart- and torpedo-stage embryos and plantlets with green cotyledons and on shoot production from them were studied in grapevine interspecific hybrids: cvs. ‘Bianca’, ‘Podarok Magaracha’ and ‘Intervitis Magaracha’. Pro-embryogenic calli were derived from petiole explants on solid full-strength NN medium supplemented with different levels of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzyladenine (BA) for each cultivar and used to initiate cell suspensions in liquid NN medium supplemented with 1 mg L-1 2,4-D and 0.2 mg L-1 BA. The following formulations of media were optimal for different stages of somatic embryogenesis and for plantlets with green cotyledons development: liquid NN medium supplemented with 0.5 mg L-1 BA  for globular embryo formation; liquid HTE medium (with PG macro-elements, thiamine and pyridoxine at 5 mg L-1 each and 0.5 mg L-1 nicotinic acid): supplemented with 0.2 mg L-1 BA  for heart-stage embryo development, supplemented with 0.1 mg L-1 indole-3-acetic acid (IAA) and 30 mg L-1 sodium humate  for torpedo-stage embryo development and supplemented with 0.5 mg L-1 gibberellic acid (GA3)  for the growth of plantlets with green cotyledons. Solid MS medium modified by the addition of nicotinic acid, pyridoxine and p-aminobenzoic acid (PAB) at 5 mg L-1 each, 0.5 mg L-1 thiamine and 0.5 mg L-1 BA was best efficient for shoot production from plantlets. Inflorescence formation in regenerated plants of cv. ‘Podarok Magaracha’ grown on solid hormone-free PG medium was induced by addition of nicotinic acid, pyridoxine and PAB at 5 mg L-1 each, 0.5 mg L-1 thiamine and 0.2 mg L-1 BA into liquid modified HTE medium for growth of plantlets with green cotyledons and into solid modified MS medium for shoot production from them.

The Makedonas is a new wine higher quality black grape’s variety, was found out by Panteley Zamanidi and Leonid Troshin in 2001 in Greece and created by hybridization between Ksinomavro and Cabernet Sauvignon varieties. It’s belong to the group of Black see pool varieties on morphologic-physiological characteristics (convar. pontica subconvar. balcanica Negr.). The variety is used for making dry red wines of excellent quality. It is very perspective variety in Southern zones of Europe where the grape couldn’t get good acidity in the juice of berries

Trapsa is a Greek native variety. It belongs to the variety group of the Black sea basin (convar. pontica subconar. balcanica Negr.) ,has limited spreading. The length of productive period is 156-165 days. Variety is medium-sized. Degree of cane maturation is high. Productivity is very high. Percentage of fruitful tillers is 90. The average mass of clusters is 500 g. It differs with high drought resistance and average resistance to fungi diseases. Under bud germination is recognized by crown of green color with winy-red shadow, with strong webby swelling. Plate of a leaf is large, dark-green shadow, form is pentagular, with five lobules, top surface of plate is honeycombed. Cluster is large, conic and compact. Flesh with nice variety after taste. Berry is average size, round, navy-black color. Sugar content is high. Variety is for making of high quality red wines of different categories in southern zones of grape-growing with hot and dry climate.

Variety Moskhoragos is hybrid one: it was bred by Panteley Zamanidi, Leonid Troshin and Leonid Maltabar in 2001 in Greece by crossing of varieties Savvatyano and Muscat Alexander. By morphological-biological characteristics it was included in a group of varieties of the Black Sea basin (convar. pontica subconar. balcanica Negr.). Variety is new and that’s why it has limited spreading. Productive period length is 156-165 days. Length of shoots is 2,1 – 3.0 m. Degree of cane maturity is high – 81-95 %. Crop capacity is 25-30 t/ha. Percentage of fruit-bearing shoots is more than 90. Average cluster mass is 300 g. It differs with high cold resistance, drought resistance and increased resistance to fungi diseases in comparison with Muscat varieties Vitis vinifera L. During blossoming it can be recognized by a little crown of greenish-yellowish color with winy-red shade in the end part, shining surface of leaves with webby downiness in the lower part. First leaf is greenish, winy-red in edges, highly downy with both sides, second and third leaves are greenish-bronze, pentalobular, slightly webby –downy in the upper side and highly downy in the lower side. Fourth and fifth leaves are green with yellowish shade, brilliant, with rare webby downiness in the upper side and highly downy in the lower side. Shoot is green, with brown-reddish shade. Leaf is symmetrical. Leaf plate surface is large, dark-green, leaf’s form is pentagonal with five lobules, bladder surface is weak. Leaf edges have middle-acuminated dens, erect by both sides. Lower lobules are slightly overlapped. Leafstalk length is average. Inflorescence is cylindrical, often with antenna. Two inflorescences usually appear on one shoot on 4 or 5 nods and inflorescences appear on the shoots, grown from resting buds on perennial wood as well. Flower is monecious, normal. Ovary is round and ball-shaped. Pollen has normal shape, fertile. Cluster is average, cylindrical, alary, with average density. Berry is middle-sized, round, yellowish-green color. Flesh is juicy, sweet, with nice Muscat after-taste. Sugar content is high. Seed has an average length, light-brown, pear-shaped, with middle-sized beak. Variety is used for preparation of high quality table, strong and dessert wines of different categories.

Peculiarities of microсlone propagation of introduced varieties and grape clones, and home varieties created in Kuban State Agrarian University as well, which were given for national trial and for industrial production are discussed in this article.

Greek variety Limniona concerns to the variety group of the Black Sea basin convar. pontica Negr. subconar balcanica Negr. It has a limited spreading, uses as an industrial variety. Duration of productive period is 156-165 days. Growth of shoots is strong. Degree of cane maturity is 81-95 %. Percentage of fruit-bearing shoots is more than 90. Productivity is 25-30 t/ha. It is highly drought resistant. Flower is bisexual. Cluster is large, cylindrical, of an average compactness with mass of 350g. Berry is middle-sized, round, dark - blue-black. Flesh is juicy, with nice variety after –taste. Variety is used for preparing of high quality red wines of different categories.

Problems of biometric evaluation of morphological features of clones for identification of variety group Merlo genotypes were considered in the paper.