Name
Troshin Leonid Petrovich
Scholastic degree
•
Academic rank
professor
Honorary rank
—
Organization, job position
Kuban State Agrarian University
Web site url
—
Articles count: 170
The article presents the results of studies of 92
indigenous varieties and 15 wild genotypes by 24
microsatellite markers. As a result a locus database about
germplasm in the collection stored at the Russian
ampelographical collection AZOSViV (Anapa Zone
Experimental Station of Viticulture and winemaking)
was created. During the research of varietal breeding
populations confirmed the validity in the production of
the cultivar of Krasnostop anapsky with obvious changes
in microsatellite sequences as a clone of famous indigenous
Don cultivar Krasnostop Zolotovskii. This clone
was selected in 1953 by prominent agrotechnologist
Zotkin Ivan Ivanovich which is propagated by the
author and his followers, and after a formal registration
submitted to state testing in 2007 in GSK RF, zoned in
the North Caucasus region (number 6) in 2014 year. Sort
-clone Krasnostop anapsky only in the Kuban today
cultivating in 35 hectares. By using molecular genetic
techniques, now clonal selection of vine grapes is reasonably
improving. Recent data collected by us in the
work process, were analyzed a comprehensive manner,
making it possible to draw conclusions about distinctness
of grape varieties. Some clones were studied by DNA
primers, based on retrotransposon sequences
The article gives the results of the study of the texture
of the grapes and biochemical characteristics of red
wine grape varieties of Cabernet Sauvignon, Cabernet
Franc, Merlot, Sapeari, Tsimladar, Pinot Noir for the
production of juice of direct extraction and blending.
The studied varieties in terms of the structure of the
cluster may be divided into three groups of blackpinene
(21.1), Cabernet Franc and Tsimladar (18.7 -
17.5), Cabernet Sauvignon, Merlot, Saperavi (16.5 -
16.8) , and bunches of addition in terms of two groups
- Tsimladar (17.6), Cabernet - Sauvignon and other
(15.6 - 16.4). The structural component of grapes varieties
ranged from 5.2 to 5.9, berry index - from 62.1 to
83.4, the output of the wort from 74.2 to 76.3%. The
content of sugars and acids in the berries of all varieties
is favorable for the production of beverages. The
largest Tartaric acid content was found in the juice of
the varieties of Cabernet Sauvignon, Pinot Black,
malic acid - in the juice of varieties Saperavi, Merlot,
Tsimladar, citric acid - in the juice Tsimladar varieties,
Cabernet Sauvignon, Saperavi. The ratio of tartaric
acid to the content of the apple juice Cabernet Sauvignon
was 2.9: 1, Cabernet Franc 2.6: 1, Pinot Black
2.5: 1, Merlot and Tsimladar 1.6: 1, 1.1 Sapevari: 1.
According to the content of potassium cations, sodium,
magnesium and calcium there were chosen three varieties
of Cabernet Sauvignon and Cabernet Franc. The
lowest content of potassium and magnesium cations
was in the variety of Tsimladar, Sodium – in the varieties
Merlot and Saperavi, calcium – in Saperavi. Excellent
tasting commended for juices was given to
Cabernet Sauvignon, Saperavi, Tsimladar, good – to
Cabernet Franc, Merlot, Pinot black. Best blending
juice obtained by mixing the juice of the grape of the
varieties of Cabernet Sauvignon and Pinot black
70:30, Saperavi and Pinot black 50:50. From the
grapes of produced varieties, we can produce juice and varietal by name ampelographic variety and blended to
extend the range
The article highlights the issues of grape plants resistance
to low temperatures; it is an urgent problem for
all wine-growing regions of the world, located in the
zone of risky agriculture. Losses caused by frost, indicate
long-standing necessity of perfection of assortment
in terms of enhancing its hardiness by enriching
more resistant genotypes obtained both through the
introduction or naturalization, as well as from the
crossing of indigenous with interspecies hybrids. Methodology for evaluating frost resistance properties
of the material served as a preparation for the originality
of his research and experimental evaluation of the
unique divisibility properties of the aim. Thus, we initially
conducted hardening of matured cuttings at positive
temperature plus 8 plus 4 ° C for 14 days (I
quenching phase) and a negative temperature minus 5 °
C-minus 7 ° C for 9 days and minus 10 ° C-1 day (II
hardening phase). Then the cuttings we frozen in the
temperature range from minus 16 ° C to the next steps
of consistent freezing (minus 16 ° C - 2 days; minus 18
° C - 2 days; minus 20 ° C - 2 days; minus 22 ° C –
1day, minus 24 ° C - 1 day, minus 26 ° C - 1 day, minus
28 ° C - 1 day and minus 30 ° C - 8 hours). After
freezing of cuttings of each variety, the sample was
placed on the 3 days in the refrigerator at the temperature
of + 2 ° C to their gradual thawing. Cuttings were
grown at room temperature on water. As a result, obtained
by the accounting: the lowest frost-resistance
among the studied forms of parental Crimean origins
was found in grades of Shabash, Soldayya and Solnechnodolinsky;
the varieties of Dzevat kara, Kokur
bely, Kokur cherny, Misket, Misgyuli kara, Sari Pandas
were set as a weak level (-20 °C); Aybatly, Kefesiya,
Kok pandas and Ekim kara have average level up
to -22 °C; the highest frost-resistance - 24 °C – was
shown by Khersonessky and Kapselsky. Also, we have
identified synthetic frost-resistant F1-population and
plus-transgressive selection form
This article presents information about the features of biotechnology as the driving force of scientific and technological progress. The national programs of the leading countries of the world, it is one of the priority sectors, reflecting the level of the socio-economic condition of the society. Biotechnology is now successfully solves such vital tasks as providing food, the establishment of effective medicaments, obtaining fuel based on renewable raw materials, maintaining ecological balance, conservation of biological resources of the Earth. The development of agriculture in modern conditions is impossible without agricultural biotechnology. It is directly related to viticulture. Choosing an object of an integrated system (embryos, apical meristem, axillary buds), it is possible to clone plants, i.e. produce plants identical to the original. If the same as the object to use isolated cells or protoplasts, in this case, there will most likely altered versions, creating diversity for the breeder. Genetic engineering – the science of younger, since the establishment of the first chimeric DNA molecule. The origin of genetic engineering is rooted in the development of molecular genetics, biochemistry. These technologies, undoubtedly progressive, but their biological safety is still insufficiently explored and is a danger to all life on Earth. The leading Western powers carried out strict control over the introduction of transgenic crop plants, as they are in agrocenosis new biological risks that may adversely affect the plants, animals and humans. In Russia, as in other countries, have already adopted the law “State regulation of genetic engineering”
The article discusses the application of automated system-cognitive
analysis (ASC-analysis), its mathematical
model is a system of information theory and implements,
its software tools – intellectual system called
"Eidos" for solving one of the important tasks of ampelography:
to quantify the similarities and differences
of different clones of grapes using contours of the
leaves. To solve this task we perform the following
steps: 1) digitization of scanned images of the leaves
and creation their mathematical models; 2) formation
mathematical models of specific leaves with the application
of information theory; 3) modeling the generalized
images of leaves of different clones on the basis
of specific leaves (multiparameter typing); 4) verification
of the model by identifying specific leaf images
with generic clones, i.e., classes (system identification);
5) quantification of the similarities and differences
of the clones, i.e. cluster-constructive analysis of
generalized images of leaves of various clones. The
specific shape of the contour of the leaf is regarded as
noise information on the clone to which it relates, including
information about the true shape of a leaf of
this clone (clean signal) and noise, which distort the
real shape, due to the random influence of the environment.
Software tools of ASA-analysis which is
intellectual "Eidos" system provides the noise suppression
and the detection of a signal about the true shape
of a leaf of each clone on the basis of a number of
noisy concrete examples of the leaves of this clone.
This creates a single image of the shape of the leaf of
each clone, independent of their specific implementations,
i.e. "Eidos" of these images (in the sense of Plato)
- the prototype or archetype (in the Jungian sense)
of the images
The article presents the material of forecasting for grape
yield of next year and establishing the optimal loading if
cutting of bushes. The material includes 14 varieties of
grapes, 11 of them are technical and 3 are table ones. For
each year of stable high yield of grapes, it is necessary to
pre-set the optimum length of fruit cutting of shoots and
optimum load on the bush healthy eyes. To do this for
each variety on the eve of trimming bushes we perform
optimum productivity analysis of wintering buds of fruit
along the length of shoots, i.e. we implement forecasting
of grape yield for next year. We have a plan of forecasting
for yields of vineyards by microscopy of wintering
buds on one-year shoots of fruit ripened grapes in order
to establish the potential of embryonic establishment of
inflorescences in the central holes of buds. Based on the
analysis of buds, the indices were calculated for wintering
fruiting buds and their degree of damage during the
growing season. It was revealed, that the majority of
grape varieties under study shows high tab embryonic
inflorescences in central buds in overwintering buds for
next year yield. Higher rates at a rate of fruiting buds
were wintering in the varieties: Moldova (section 27). -
1.66; Bianca (section 6). - 1.83; Kunlean (section 15). -
1.71; Merlot (section 14). - 1.64; Saperavi (section 56). -
1.76. The lowest rates of fructification - the varieties
Muscat Hamburg (section 21) and Augustine (section
11) and were respectively 1.20 and 1.24. As a planned
productivity, we offered the optimal loading model of
cutting bushes buds. As a result of productivity analyzes
of buds along the length of the fruit shoots in 2016 we
recommended to carry out pruning of fruit annual shoots
3-4 buds of the form of AZOS-1 and the form of cordon
- 5-6 buds
Peculiarities of breeding oidium-resistant genotypes in
crossing Crimea autochthonous grape varieties with
complex interspecific hybrids were studied. Twentytwo
populations consisting of 1323 seedlings were
analyzed over a period of 2008-2014. The highest
oidium resistance was registered in the progeny of the
cross Khersonesskii х ЖС 26-205 (6.8 points), and the
average estimate of oidium resistance across all the
study populations was higher than that of the initial
autochthonous grapes of the Crimea. The level of
hypothetical heterosis in the majority of the study
populations indicates that, on the whole, the hybrid
seedlings possess a higher oidium resistance relative to
the initial Crimea autochthonous grapes. The highest
breeding value was associated with cross combinations
having the complex interspecific hybrids Muscat JIM,
Spartanets Magaracha and Magarach 31-77-10 in their
pedigrees. The cross combination Muscat JIM х
Shabash was the most efficient, yielding 10.7 per cent
of oidium-resistant seedlings. It was reliably
established that a high level of the genotypically determined inheritance of oidium resistance is found
in crosses with the participation of the interspecific
forms Magarach № 31-77-10 and Muscat JIM (female
parents) and Spartanets Magaracha and Tsitronnyi
Magaracha (male parents). The expediency of this
direction of breeding research was proved
Progress of breeding for resistance to diseases, pests
and low temperatures is provided by cross-species
hybridization. Institute of Vine and Wine "Magarach"
is one of the leading centers of selection of grapes in
the world. The breeding program of deducing grapes at
the institute is based on the study and attracting the
world's gene pool, including and industrial of the
Russian Federation assortment of grapes. Analysis
assortment shows that the share of productive, highquality
varieties with group resistance to diseases,
pests and extreme environmental factors is very
small. The main method of selection of grapes of a
new generation who have genetically determined traits
earliness, resistance to biotic and abiotic factors is
crossing complex interspecific hybrids and forms of
their involvement with each other and with the forms
of species Vitis vinifera, belonging to different ecogeographical
groups, saturating crossing between
hybrid forms with complex features, the appropriate
selection of reference. For vigorous offspring is most
effective as a parent to use a local variety of forms of
Crohn and father - interspecific cultivar Alminsky
The article presents the results of the studies of 11
varieties cultivated in the eastern region of South Coast
wine-growing zone of Crimea (planting of
"Solnechnaja Dolina ", near Sudak mountain) in the
area of their natural growing, where was formed the
gene pool of grapes for a long time. As a result, the
three-year (2010-2012) study found that the majority
of indigenous varieties had small number of fruitbearing
shoots in the bush: the varieties with colored
berry - 53,3-73,3%, in white-berry - 46,6-75,9% except
Shabash variety - 79.9%. We have identified the most
productive varieties which were Dzhevat kara (62.2 t /
ha), Kokur Beliy (48.9 t / ha), Kapselsky Beliy (44.4 t /
ha), and varieties with female type of flower: Kefesiya,
Ekim kara, Kock pandas, Sary pandas and Crona gave
not high yield: 22.2; 22.2; 24.4; 28.9 and 28.9 t / ha.
On the strength of growth, productivity and the
accumulation of sugars in the juice of berries, we
picked out varieties of Dzhevat kara, Crona, Kapselsky
beliy. According to the main economic indicators, it
turned out that the most profitable varieties are
Dzhevat Kara, Kokur beliy and Kapselsky beliy.
Hence, these varieties in conditions of the Crimea have
much greater prospect of reproduction and
development of production
The article presents historical information about the
stages of gradual transformation of the first non-large
wine-making institution of Crimea, organized in 1828
under the initiative of Prince M.S. Vorontsov in the
natural boundary called Magarach near the Imperial
Nikitsky botanical garden in the world famous Institute
of Grape and Wine "Magarach" - once the chief
coordinator of the research work in the field of viticulture
and winemaking republics of the USSR and the
main forge of scientific staff in this direction. Among
the directors of the institute of the last century, there
were such talented organizers as A.G. Globa, T.G.
Kataryan, P.Y. Golodriga. The Institute operated a
network of regional branches, created enotec with a
settled in 1836 wine Muscat pink Magarach (World
rarity is reflected in the Guinness book of records), the
world ampelographic collection (fourth in number of
samples), scientists developed area-standard assortment
for production, issued 11 Volumes of "Ampelography
of USSR" (awarded by OIV), designed a lot
of outstanding for yield and stability varieties (Pervenets
Magaracha, Ranniy Magaracha, Citron
Magaracha et al.) and clones (Muscat white of Red
Stone, Pinot Noir yields and others.) grapes. There
was a significant contribution in the sphere of research
and development of viticulture and winemaking of
world, the institute was given an international prestige
and this led to establishing the base of the General
Assembly of the OIV - International Organization of
Grape and Wine, the International Symposium on the
selection of wine-town, several international wine
tasting competitions and International finest examples
of table grapes