The influence of reduced iron nanoparticles and its oxide Fe2O3 on the growth rate of Chlorella vulgaris IFR # S-111 added to the Tamiya nutrient medium is studied. In the range of concentration of additives 0-0,1 g/l (gram per litre) an oxide inhibited the growth of microalga already when its content in the environment 6,25*10-3 g/l. With the increase of reduced iron concentration in the first twenty-four hours in the whole range the growth of the cell concentration of microalga was observed. With a maximum iron concentration of 0,1 g/l the density of a chlorella exceeded the control for 70%, and in 48 hours for 150%. The microscopy hasn't shown morphological changes of a chlorella cells with addition of the nanoparticles to nutrient medium. Accelerated reproduction of the microalga cells became the result of the intensification of the photosynthetic processes, as indicated by the nature of the parameters of delayed fluorescence (DF) of chlorophyll and shapes of the induction curves. The maximum level of DF in all experimental samples after 24 hours of cultivation was significantly higher than the control, and photosynthetic activity increased with increasing concentration of nanoparticles. In 48 hours the maximum activity was observed at concentration of nano iron 0,0125 mg/l, then decreased. It shows that with an intensive growth of a chlorella the potential of nutrient medium was quicker exhausted that led to decrease in intensity of photosynthetic processes
The work was done in the research laboratory of the
department of Biotechnology, Biochemistry and
Biophysics of Kuban State Agrarian University, the
aim of which was to select the most appropriate
nutrient medium for maximum growth of lactic acid
microorganism growth. The object of the study was its
own microflora of gastrointestinal tract of quails ‒
Lactobacillus agilis, Lactobacillus intermedius and
Lactobacillus salivarius. As a nutrient substrate there
were used 4 of the nutrient media: the medium for
lactic acid bacteria (city Uglich), the melasse-autolysis
medium, the glucose-peptone medium and the MRS.
During the cultivation of the microflora of the variable
parameters were the time and temperature of
cultivation. During the cultivation of microflora the
time and the temperature of growing were the variable
parameters. In the process of growing there was
carried out the analysis of dynamics of consumption of
reducing substances and titers of microorganisms. According to the results of growing of microbial
cultures there was revealed the active consumption of
carbon substrate in used variants of nutrient media,
and it was found that there was observed the maximum
of cells to 24 h of cultivation. On the basis of carried
out results of cultivation on different media and at
different parameters there was determined that the
most titer of cells was reached to 24 h at the
temperature 38,0 °С on the melasse-autolysis medium.
So, the melasse-autolysis medium can be
recommended in production conditions as the cheaper
substrate at the further development of biological
preparations for poultry breeding
The article shows the influence of associated species on oak trees in small groups and evaluation of the state of the oak tree category depending on its surrounding
The review examines the recent years’ data
pertaining to the interaction of plants with
nanoparticles of noble metals (gold and silver) and
with nanoparticles of magnesium as an element that
has a central role in the organization of the cellular
protein-synthesizing apparatus. Information is
presented about the effect of gold and silver
nanoparticles on plants and on possible nanoparticle
phytotoxicity. It is shown that magnesium
nanoparticles, as well as those of noble metals,
produce various effects on the growth and
productivity of plants, enabling the arsenal of
biologically active substances to be expanded.
Analysis is made of the involvement of magnesium
nanoparticles in cryobiological reaction of the
regular breakdown of plant RNA under conditions of ultra low temperatures, which is important not
only for the further developments of views of the
origin of life ob Earth but also for the purposes of
designing new markers for the analysis of the
biological peculiarities of agricultural crops on the
basis of studies of magnesium in the long-lived
RNA of their mature seeds. The presented critical
analysis of the literature data, which are often
incomplete and contradictory, suggest that a
coordinated research program is needed that would
detect correlation between particle parameters,
experimental design, and observed biological
effects
Correlation interrelations of some morpho¬logical signs of winter wheat and their influ¬ence on productivity are established. Positive correlation communication of efficiency with weight of grain with an ear (r=0,70) is revealed
The results of introduction test of species and cultivars
variety of irises in the Central Yakutia are described in
this article. Climatic conditions of the introduction
region are extreme and they are strong restrictive
factor at introduction to the culture of many other
species and cultivar of decorative plants. The condition
of plants after rewintering was estimated on a 4-mark
scale. Total number of the plants which were lost in
the first winter (0 points) is 126 species and cultivars
that is 60,9%. For 01.06.2015 year a collection of
irises in Yakutia makes 40 exemplar of species (22
species) and 31 exemplar of cultivars (30 cultivars), 7
species of them are successfully adapted (3 points)
(Iris biglumis Vahl., I. bloudowii Ledeb., I. potaninii
Maxim., I. scariosa Willd., I. setosa Pallas ex Link.,
I. tigridia Bunge, Pardanthopsis dichotoma (Pallas)
Lenz). The listed species annually plentifully blossom,
form seeds and intensive form escapes. Four species
Iris biglumis, I. scariosa, I. bloudowii, Pardanthopsis
dichotoma are form stable self-seedling
Clonal micro-multiplication – is this the basic method
of obtaining the qualitative virus-free planting
material. The high quality specificity of the explants
reaction of the grapes varieties to the composition of
nutrient medium requires the individual selection of
the medium components for the most successful multiplication
in vitro. In the article we present the results
of studies on the cultivation of the grapes apexes
in vitro the modified medium with the reduced
content of macrocells. As a result of conducted investigations
we have established that for the grapes varieties
of Academic Trubilin, Artemis, Gurman
Kraynova, Maria Kallas, Nizina, Petit Verdo and
Traminer Black the most effective introduction into
the in vitro culture (acclimatization of apexes 80-
100%) occurs on modified nutrient medium to
Murasige and Skoog (1962), by being differed in
terms of the content macro-elements (mG/l):
NH4NO3 – 1237; KNO3 – 1425; MgSO4 ·
7N2O – 277,5; KN2PO4 · H2O – 277,5, of the
vitamin Of v1 – 10,0 mG/l, nicotinic acid – 4 mG/l.
Remaining varieties of Kishmish luchistiy, Preobrajenie,
Roshfor K and Yubiley Novocherkasska in the
same medium were developed slowly and in a different
way
The flora of Yakutia is rich in medicinal plants. Many
of these plants are not still studied totally. The species
Rhodiola (Crassulaceae) is one of the most popular
family which are widely used by the local population
for therapeutic purposes. Medicals use the rhizome and
roots of Rhodiola rosea as a source of medicinal raw
materials. This means the removal and destruction of
plants. The procurement should be carried out solely
on the basis of plants grown in culture because of the
rarity and few natural reserves of the species. R. rosea
L. passes a full cycle of the sprouts, blossoming and
bearing fruits during the introduction in the conditions
of Central Yakutia. The weather conditions of the year
influence on the rate of development and growth
processes very strong. The experiments determination
of the germination of local reproductions were
negative. The sprouts grow very good. In this case, it is
good possibility of intense vegetative reproduction of
Rhodiola. The evaluation of stability of introduction
may be attributed to the kind of group stability. The
great potential and adaptive capacity of R. rosea
significantly enhances the possibility of growing this
species under conditions of the Central Yakutia. It is
recommended to improve and elaborate of protection
of natural populations and unique plant communities
There were developed some methods of sterilization and introduction of the following endangered species of Caucasian flora to in vitro culture: Campanula sclerophylla Kolak., Lilium caucasicum Miscz. ex Grossh., Daphne woronowii Kolak., Pancratium maritimum L.. The effect of different plant hormones as well as their combinations and concentrations on the regeneration, growth and development of plants is studied. As a result of clonal propagation, more than 100 plants of Campanula sclerophylla Kolak, about 30 plants of Pancratium maritimum L., and 150 samples of Lilium caucasicum Miscz. Ex Grossh are saved in tissue culture
The article gives an analysis of the elemental
composition in raw materials of stevia. Stevia plant is
able to regulate the flow of heavy metals, allowing
them, on the one hand, actively oppose the excessive
intake of toxic elements and on the other hand,
selectively cumulate essential elements. The contents
of essential elements in plant raw materials do not
exceed the acceptable standards regardless of the
growing conditions