The problem of increasing resistance of winter
barley to adverse conditions of winter is the most
problem in the selection area of this crop. The main
component of this complex trait is the frost
resistance. Laboratory methods of assessment of
frost resistance are important for breeding of winter
barley. These methods can effectively replace more
costly method of direct freezing in the freezers.
Magnesium cations (Mg++) are essential
components of the protein-synthesizing system of
germinating seeds. Magnesium cations determine
the stability and translational activity of mRNA and
rRNA. Trilon B is usually used for in vitro banding
of magnesium cations in area of biochemistry of the
nucleic acids. It was very interesting to verify the
Trilon B influence to seed germination of winter
barley varieties by experiment. Experiments
showed: actinomycin D – the inhibitor of RNA
synthesis – didn’t show varieties specific effect to
seed growth of winter barley, while Trilon B had a
varieties specific impact to length coleoptiles and
roots. Research was carried out on etiolated
seedlings at temperatures from 22 to 28oC, and different concentrations of Trilon B - 1,6×10-3Ðœ,
2,4×10-3Ðœ and 3,2×10-3Ðœ. The roots of 3-days old
seedlings were more sensitive to Trilon B influence.
It is shown the varieties of Russian origin were
naturally reacted to Trilon B: the higher the frost
resistance of variety, the more resistant variety to
the action of various concentrations of Trilon B at
different temperatures. The most optimum
concentration was 3,2 × 10-3Ðœ at 22-25oC.
Varieties by foreign origin deviated from this
pattern. It is interesting to research the genetic
essence of these differences. It will be helpful for
optimization of varieties differentiation conditions
to create a laboratory method of estimate of frost
resistance of winter barley. The new method will be
most efficient compared the direct freezing method
We propose a method of biodiagnostics of soil resistance to chemical pollution and its regulation on the degree of violation of the ecological functions of soil based on the definition of the integral indicator of biological state (IIBS) of the soil. The comparative assessment of the stability of the major soils of the south Russian to chemical pollution are considered in this article
The differential stability of mRNA is an important
mechanism for posttranscriptional regulation of gene
expression in eukaryotes. Messenger RNA stability is
controlled by specific genes and growth conditions.
The review examines the theoretical possibility of
mRNA stabilization in vivo as a consequence of
replacing carbon atoms (C) or phosphorus (P),
composed of nucleic acid on the silicon (Si) atom.
During isolation of poly-(A)+mRNA from plant
tissues by the two-cycle affinity chromatography on
poly-(U)-Sepharose, regular changes in poly-
(A)++mRNA yield were observed. The changes
varied both with the plant genotype and growth
conditions. Celite treatment of heated and unheated
total RNA preparations from developing corn kernels
and from green and etiolated wheat seedlings.
Messenger RNA that differed in the length of poly-
(A)-sequences was used for hybridization. It is
evident that a reduction of poly-(A)-length causes
alterations in spatial structure of mRNA, and
associated proteins and cations Mg++ become
accessible to celite absorption. Heating promotes
melting of secondary structure, already initiated, and
increases the efficiency of mRNA stabilization by
celite. Interpreting the facts interact celite with mRNA
in vitro and stabilization of mRNA in vivo by
cycloheximide with a modern point of view can be considered with the position research of the
phenomenon of RNA interference
There has been conducted the analysis of woody plants
quantity in various types of stands in Rostov-on-Don,
as well as the evaluation of their state and the rate of
environmental resistance destabilization.
It has been ascertained that the stands of almost all of
the urban gardening objects are in a salvaged
condition. There have been elaborated
recommendations for enhancement of the stands
inspected
The river Chelbas – is the steppe river passing through all Chelbasskaya village of the Kanevsky area. Selting of small rivers leads to rise in level of ground waters and bogging of flood plains which become unsuitable for any use. Harmful influence can conduce coming in a reservoir of a large number of suspensions. Suspensions increase water turbidity, reduce depth of penetration of sunshine, i.e. reduce a layer in which there is a photosynthesis that conducts to decrease of primary production of a waterway and deficiency of oxygen. The increase in ground rainfall can lead to change of fauna of a benthos, a silting of spawning areas, death from suffocation of have already spawned caviar of fishes. Research of the right river bank of Chelbas in the territory of stanitsa Chelbasskaya included studying of qualitative structure of wood, shrubby and grassy vegetation of a coastal strip, visual assessment of its state and definition of the general projective covering. In the lower current of these rivers the mineralization raises to 3-5 g/l. It speaks, apparently, by that the water containing alluvial deposits in the top parts of valleys have higher filtrational properties better are washed out, than on surrounding watersheds and slopes, and also down valleys. Works on bank protection will improve a condition of a right-bank strip of river Chelbas
Qualitative and quantitative composition of the low-molecular carboxylic acids (CA) which were contained in the cultural liquid (CL) of the four strains of Escherichia coli and Salmonella enteritidis var.Issatchenko was investigated. The differences in the composition of the CA were shown, their dynamics during the bacterial growth and their total concentration in CL were strain specific characteristic. The differences in the composition and dynamics of the CA, which are specific only to probiotic and opportunistic strains were revealed
The article presents the structural and phylogenetic
analysis of VIT_02s0033g00410,
VIT_02s0033g00390 and VIT_02s0033g00450
genes of the grapevine genome and the closely
related orthologous gene MYB114 of the
Arabidopsis genome. These genes are responsible
for the biosynthesis of anthocyanin in the organs of
model plants and are of interest not only for practical
production and breeding, but also for fundamental
research. These genes were analyzed for GCcomposition
of nucleotides, the presence of cisregulatory
elements and promoter regions. DNA and
protein sequences were aligned to look for similar
elements, which allowed further analysis of the
ultraconservative domains of four genes. Based on
the results of search and identification of the
conservative regions, a cluster tree was constructed,
which made it possible to identify the separation of
gene sidelines from, presumably, the main one. At
the same time, the construction of consensus trees
based on DNA and protein sequences revealed their
absolute similarity. "The Minimal Evolution Tree"
allowed calculating the approximate dates of the
appearance of the mutations and the divergence
times of the gene branches between each other. At
the same time, the appearance of the Vitis genus and
its separation from the Rosales was taken as the time
first divergence point. In the end, homologous
metabolic pathways were searched between
grapevine and Arabidopsis, which revealed the
presence of homologous proteins in the grape
proteome. In this turn, it already confirms the
existence of similar biosynthetic pathways and, as a
consequence, interactions such as "DNA-protein"
and "protein-protein"
The article presets results of the studies that reflect the nature of the distribution of forests of Predkamje of the Republic of Tatarstan on the types of forest grown conditions, the predominant tree species, age, site class, completeness and volume of stem wood. It is shown that there are large reserves of increasing productivity of forest stands by optimizing their density and species composition of forests
Use of algae as bioindicators on soil pollution by xenobiotics was studied in Krasnodar territory. There was diagnosed the low specific content of soil algae on the territory of a pesticide warehouse. There were presented some species, characteristic for the polluted soils.
Algae as bioindicators on soil pollution by xenobiotics were studied in Krasnodar region. Explicit alteration of algacenosis was observed on the territory functioning petroleum production site, it testifies the consistent soil pollution. Alga flora of preserve area zone differentiated with high species diversity.