Due to the fact that in floodplain lands there is instability in the yield of agricultural crops because of flooding and waterlogging, protection of the territories is necessary. The area of the foothills of the Kuban River, where the reclaimed field is located, was chosen as the object of study. Since this territory is subjected to periodic flooding, and in order to preserve the fertility of this soil, a set of measures has been taken. Hydrometeorological and hydrogeological studies are used as the main method. The parameters of the dam and drainage system were determined, the locations of the regulatory structure and the location of the observation wells were substantiated. To assess the measures taken, soybean was grown on the field during the period of greatest rainfall. After sowing, soil samples were collected at three points and conducted research aimed at the influence of the drainage system. It has been established that the complex of measures influences the meliorative state of the field for the better. In the area near the main collector, the soil is most depressed after the processes of flooding and waterlogging, the seedlings of the soybean plants are sparse, and by the middle of the field the plants developed evenly and had almost identical morphometric indicators. Were obtained biometric indicators in different parts of the soil, which are confirmed by experiment
In the review article we present a comparative analysis of frost resistance of varieties of winter barley and sunflower (Helianthus annuus L.) by the results of freezing in the refrigerating chambers and by the hygroscopicity of mature grain. On the example of a number of varieties of both cultures, it is shown that the higher the frost resistance of the variety, the smaller the volume of the supernatant can be obtained by extracting the press cake with a solution containing magnesium cations. It is assumed that increased hygroscopicity of winter barley and sunflower press cake is associated with a relatively high content of so - called "water-soluble starch" - β-glucan polysaccharides in its grain. The possibility of regulation of the degree of hygroscopicity under the influence of potassium (KNO3), zinc (ZnSO4) and gibberellic acid (GA3) was studied on a number of sunflower varieties. It was shown that treatment with a solution of potassium (a concentration of 50 ppm KNO3) of sunflower plants at the stage of 4 leaves leads to a significant increase in hygroscopicity of grain and hardiness of plants, little effect was given treatment with zinc (30 ppm ZnSO4) and a lesser effect was observed when treating plants gibberellovaja acid (20 ppm). Potassium treatment significantly intensified the biosynthesis of free Proline, phenolic compounds and soluble proteins. When processing zinc significantly increased the content of carbohydrates in sunflower plants. Hygroscopicity showed a significant correlation with the content of free Proline (R2=0.621), phenolic compounds (R2=0.907), total carbohydrates (R2=0.673) and soluble proteins (R2=0.708). It is assumed that the treatment of plants with potassium and zinc plays a key role in increasing the hygroscopicity of grain. The results of comparative studies of frost resistance on the degree of survival of plants during freezing in cold rooms and the degree of hygroscopicity of mature grain showed that these two methods of assessing frost resistance give very close data. At the same time, in terms of simplicity and low economic costs, the proposed method of assessing frost resistance is many times superior to the method of direct freezing of plants
The article presents results of the research on the
chemical composition of cognac wine materials,
distillates and aged alcohols from the varieties of the
Magarach Pervenets and Rice of different sugar
content grown in the Taman agroecological zone. The
influence of the initial sugar content on the chemical
composition, physicochemical parameters and
organoleptic evaluation, as well as the correlation of
soil-climatic conditions and varietal features on the
composition of cognac distillates, is shown. Soil
analyzes were carried out at the FGBIC Anapskaya
ZOSViV SKZNIISiV with the use of the latest
equipment by conventional methods of determining
indicators of soil characteristics. The purpose of the
research is to give a detailed description of the site
under study, as well as to identify the boundaries of
the ecological macrozone for cultivating cognac
varieties of grapes. In the course of the research it was
clarified that for the selection and evaluation of raw
zones of cognac production, it is necessary to take
into account the optimal soil and climatic parameters
for obtaining high-quality cognac raw materials
As a result of 12-year research, we found the influence of management and control factors on the population of pests and disease damage of four varieties of winter wheat in KNIISH of P.P. Lukyanenko in order to optimize the plant protection factor. The quantitative characteristics of populations of pests and diseases essentially depend on the agronomist and weather predictors. It has been established that for 8 years the population of winter wheat by the larvae of cereal leaf beetle was higher than the EPO and the protection has been important economically and agro-politically. The maximum population of spikes of wheat thrips was observed in 2009, 2010, 2013 and 2014 and amounted to 24.2-35.3 specimens / spike, which was the basis for the use of insecticides. With optimal weather predictors in 2010, 2011, 2012 and 2013, the population of spikes with larvae of wheat mosquito (without plant protection) was from 15.3 to 19.0 specimens / spike, which is higher than the EPO. Monitoring of the dynamics the development of diseases allowed conclusion that the state of the population of pathogens is limited by the levels of fertility and mineral nutrition and weather predictors. The isolation of the values of these indicators in different periods of development of pests allows to optimize the implementation of operational measures with the contribution of the plant protection factor to the productivity of winter wheat from 7 to 42%
Agronomy systems with good reason can be
considered as complex multiparameter natural and
technical systems. In these systems, there are
numerous and diverse physical, chemical and
biological processes. On the one hand, these processes
have a significant impact on the performance of these
systems. On the other hand, they are extremely
difficult to be described in the form of meaningful
analytical models based on equations. As a result, the
development of meaningful analytical models is
associated with a large number of simplifying
assumptions that reduce the validity of these models.
Usually we consider linear univariate models for
agronomic systems, whereas practices are necessary
for nonlinear multiparameter models. Thus, we face
the problem proposed to be solved by the application
of a phenomenological meaningful systemic cognitive
models. These models are created using automated
system-cognitive analysis (ASC-analysis) using the
intellectual system called "Eidos" directly based on
empirical data and used for the decision of tasks of
forecasting, decision support and research of the
modeled subject area. In this case, empirical data can
be large, incomplete (fragmented), noisy, presented in
different types of measuring scales (nominal, ordinal
and numerical) and in different units of measurement.
The comparability of the processing of heterogeneous
data is ensured by the fact that they are all converted
into units of measurement of the amount of
information. A numerical example has been given
This overview and theoretical article deals with the consideration of hypothetical possibilities for the development of molecular-kinetic markers of agricultural plants allowing to quantify the effect of genotype-environment interaction on the basis of stability studies of mRNA. The development in view is based on the results of studies of the mRNA decay identity in vivo and in vitro (the ommp system), as well as the phenomenon of RNA interference (RNAi), widely studied in plants. The ommp system has allowed to establish the relationship of the cultivar-specific growth reaction to the effects of low positive temperatures, dehydration, salinity, illumination and biologically active substances with the stability of the total and a number of gene-specific mRNAs of green and etiolated seedlings of winter soft wheat (Triticum aestivum L.) and winter barley (Hordeum vulgare L.). Similar studies of mRNA stability have been conducted on ripening grains of maize (Zea mays L.), with a particular result of the in vivo and in vitro decay identity confirmed for major mRNAs of stored proteins, 19 and 22 kDa zeins, of normal maize and mutant according to the regulatory gene opaque-2, changing the amount and stability of zein mRNA in the maturing grain of high-lysine maize. Regulatory response of the organism through RNAi is also multiple and includes neutralization of viral and bacterial infections, reaction to pathogens and biologically active substances, circadian rhythms, water stress, hypoxia, mechanical stress, mineral nutrition, salt stress and temperature changes. Unfavorable environmental influences result in an increase or decrease in the expression of certain microRNAs (miRNAs). The change in mRNA stability is an important component of the gene expression regulation system in eukaryotic cells. The main determinants of mRNA stability are in the 3'-untranslated region. It is the It is the (U)nА sequence and the degree of polyadenylation of mRNA, i.e. length of its terminal homonucleotide chain. It is to this region that mRNA molecules are complementary to miRNA. The most important component, which largely determines the regularity of the genotype-environment interaction, is the polyadenyl sequence at the 3 'end of the mRNA. Its length depends both on the genotype and on the environmental conditions. There is evidence that polyadenylation degree of mRNA determines the secondary structure of the molecule. As is known, deadenylation of mRNA reduces its lifetime, and when the poly-A-tail reaches several dozens of nucleotides, an explosive disintegration of the mRNA molecule occurs. Consequently, in a living cell the following mRNA decomposition scheme appears to be logical: shortening the poly-A-tail of the mRNA opens the sites of miRNA interaction with the 3'-non-coding region of the mRNA molecule, which causes its degradation. Thus, it is safe to assume that the interaction of mRNA and miRNA in the ommp system is the underlying process for molecular-kinetic markers under development
The article presents the results of a study to determine the changes in fertility in the typical black soil of the Belgorod region with different technologies of maize cultivation in seed farming
In cherry breeding aimed to improve fruit quality,
methods of intervarietal, interspecific and remote
hybridization are often used. As a result of the
conducted studies, new promising cherry varieties of
different maturation period, developed in the
conditions of the Lower Volga region by the method
of remote ecologic-genetic hybridization using the
best local varieties, were studied. The most effective
crosses are those developed with use of high-quality
cherry forms Zhukovskaya and Podbelskaya.
According to the aggregate parameters of fruit
quality (dry matter content, sugar-acid index), the
following varieties were positively identified:
Temnookrashennaya, Dubovochka, Lydia,
Rozovaya, Lyubimitsa, Coloritnaya, Korneevskaya,
Loznovskaya, Afina, Sharada, Lexema. The content
of vitamin C - Temnookrashennaya, Lydia,
Coloritnaya, Rozovaya, Lexema. Varieties
Temnookrashennaya, Lydia, Coloritnaya, Rozovaya,
Lexema are offered for use in the canning industry
Isolation of high-quality RNA from the tissues of perennial woody plants, including woody grape vines, is very difficult due to the high content of phenolic compounds, secondary metabolites and polysaccharides and the ribonuclease activity of destroyed tissues. Most of the existing methods require either large time or financial costs, or do not give reproducible results in the case of RNA extraction from mature grape tissues. The modified isolation protocol is based on a combination and modification of the known RNA extraction methods, taking into account the characteristics of mature grape tissues. Existing commercial kits for the isolation of RNA from plant tissues showed a low efficiency of RNA extraction from mature grape tissues, primarily associated with "varietal specificity". Reproducible results in the extraction of RNA showed CTAB-method, however, it has several significant drawbacks associated with the duration of the extraction and the complexity of the processing of an RNA preparation with a DNAase. The developed method is based on increasing the concentration of mercaptoethanol and polyvinylpyrrolidone in the extraction buffer, eliminating the stage of RNA selective precipitation via LiCl, and replacing it with deposition on a silica-based membrane (SiO2) followed by processing with DNA-ase. and increase the purity of the preparation of RNA from genomic DNA in comparison with the original method. A modified isolation protocol was developed based on a combination and modification of known RNA extraction methods, taking into account the characteristics of mature grape tissues. This solution allows to obtain reproducible quantity and quality of RNA for the subsequent synthesis of cDNA and RT-PCR
This article discusses the results of a study to determine the impact of different primary tillage to sunflower yield. The task of the research was to determine the impact of primary tillage in the agrophysical and agrochemical soil properties. It has been established that the content of productive moisture during the growing period in 0-100 cm soil layer does not depend on the method of processing. Surface treatment of the soil did not have negative effects on soil properties and its agrophysical structure in comparison with moldboard ploughing. . When dump processing improves the density of the soil, accumulates more moisture in the autumn winter period, reduces the weediness of sunflower perennial weeds. Productivity in contaminated areas increases by 3.3 - 5.4 c / ha. On weed-free crops, the yield increased by 2.5 - 3.5 c / ha, due to the improvement of the physical parameters of the soil