Meat Academy website http://meatinfo.ru has a comparative table of breeds of cattle on 8 indicators, from which 2 are text and 6 are numerical http://meatinfo.ru/info/show?id=197. It is a natural question for business executives, which of these breeds are similar throughout the system of indicators characterizing them, and which ones differ and to what extent. There is also the question of which indicators are similar and different in meaning and by how much. This article is devoted to the solution of these problems. The results of the study can be used by anyone, due to the fact that Eidos the universal automated system, which is a tool of ask-analysis, is in full open free access on the author's website at: http://lc.kubagro.ru/aidos/_Aidos-X.htm, and numerical examples of solving the mentioned problems with the use of artificial intelligence technologies are placed as a cloud Eidos-application #131
The article discusses the organizational scheme of sugar beet seed production, as well as technological methods of seed production using the stick method and the cultivation of basic seeds in NRU. We substantiate the key elements of the cultivation of uterine sugar beet of summer sowing season
Isolation of high-quality RNA from the tissues of perennial woody plants, including woody grape vines, is very difficult due to the high content of phenolic compounds, secondary metabolites and polysaccharides and the ribonuclease activity of destroyed tissues. Most of the existing methods require either large time or financial costs, or do not give reproducible results in the case of RNA extraction from mature grape tissues. The modified isolation protocol is based on a combination and modification of the known RNA extraction methods, taking into account the characteristics of mature grape tissues. Existing commercial kits for the isolation of RNA from plant tissues showed a low efficiency of RNA extraction from mature grape tissues, primarily associated with "varietal specificity". Reproducible results in the extraction of RNA showed CTAB-method, however, it has several significant drawbacks associated with the duration of the extraction and the complexity of the processing of an RNA preparation with a DNAase. The developed method is based on increasing the concentration of mercaptoethanol and polyvinylpyrrolidone in the extraction buffer, eliminating the stage of RNA selective precipitation via LiCl, and replacing it with deposition on a silica-based membrane (SiO2) followed by processing with DNA-ase. and increase the purity of the preparation of RNA from genomic DNA in comparison with the original method. A modified isolation protocol was developed based on a combination and modification of known RNA extraction methods, taking into account the characteristics of mature grape tissues. This solution allows to obtain reproducible quantity and quality of RNA for the subsequent synthesis of cDNA and RT-PCR
In the article we present the analysis of the results of studies of russian, foreign scientists, as well as our own data, obtained in the clonal micro-multiplication and sanitation of the plum domestic from Sharkey's virus (PPV). The urgency of work is determined by the fact that the plum is the second on the significance culture in horticulture of the south of Russia. One of the most economically significant objects of virus etiology on the discharge of relative is considered to be Sharkey's virus of plum (Plum pox potyvirus). In the Krasnodar region and in the Russian Federation for the first time, Sharkey's virus of plum was discovered in the end of the 1980th, after which it widely extended into other regions of the country. In the complex of the methods of sanitation from Sharkey's virus of plum we successfully use thermo- and chemotherapy in combination with the method of apical meristem. The mechanism of the formation of virus-free meristem consists of the delay of the process of the replication of virus particles from the rapid, anticipating increase in the rudimentary cloths and organs, especially, if plant undergoes by thermo- or chemotherapy. As virucide there are used the preparations neo -DHt (85 mG/l), and also salycilic acid in the concentration of 3kh10-4 M with the simultaneous magnetic-pulse working of mericlons. As the most suitable nutrient medium for the micro-multiplication of plum we considered Murasige- Skoog medium, on basis of which we have prepared different modifications. As the growth factors, which increase the effectiveness of multiplication, we used 6-BAP in the concentration 0,5-1 mG/l (in the stage of introduction into the culture and the stage of animated cartoon). For the improvement in the quality of the micro-shoots of plum, additionally to the growth factors, one should apply succinic acid, succinates of potassium and sodium in the concentration 4 mG/l. Obtained in the course of clonal micro-multiplication and adapted mericlons will be tested to the virus carrying ability and will be approved according to the quality signs. The ovaries of initial plants are embedded by healthy quality seedlings
Great success in increasing rice production, and it has more than doubled, has occurred worldwide thanks to the use of modern high-yielding rice varieties developed by conventional breeding methods. In order to develop a new variety, it is important for the breeder to study the genetic and breeding value of various cultural and wild species - carriers of economically valuable traits, the identification of genes and groups of genes controlling the economically valuable traits, to study the patterns of their inheritance, to establish patterns of interaction between the genotype and the environment, explore the genetic and physiological-biochemical basis of the phenomenon of heterosis, improve methods of intraspecific, complex, stepwise and distant hybridization, to study a phenomenon of a sharp increase in the quantitative characteristics of crop plants, a more powerful growth of hybrids of the first generation compared with the parent individuals. Scientists of the past centuries, such as A.F. Wigman, W.Noden, V.Foke, and many others have been studying the phenomenon of hybrid power, which was first named “heterosis” in 1908 by J. Schell. At present, heterosis is understood as the phenomenon when hybrids of the first generation exceed their parental forms in terms of their biological values and economically valuable traits. The magnitude of heterosis of the first generation hybrids is determined by several methods in terms of percentages to the paternal, to the maternal form, to the average of both parents, and to a better or regionalized standard. By the magnitude of the dominance coefficient, researchers often determine the inheritance of a trait
The researchers obtained data on the morphological, biochemical and trace element composition of the blood of bull-calves of Hereford breed of different sizes. In this regard, scientists and business executives have three natural questions: 1) whether it is possible to predict the size and thus the meat productivity of bulls using these blood indicators; what are the strength and direction of the influence of certain values of blood indicators on the size and weight of bulls; what blood indicators are similar in meaning, and what are different and how much (to what extent). The article is devoted to the reasoned answers to these questions by applying modern methods of mathematical and numerical modeling to solve the corresponding problems. The results of the study can be used by anyone, due to the fact that Eidos the universal automated system, which is a tool of ask-analysis, is in full open free access on the author's website at: http://lc.kubagro.ru/aidos/_Aidos-X.htm, and numerical examples of solving the mentioned problems with the use of artificial intelligence technologies are placed as a cloud Eidos-application #133
The article presents the results of a study to determine the changes in fertility in the typical black soil of the Belgorod region with different technologies of maize cultivation in seed farming
The article considers the data on the applicability of bioinsecticides based on the granulovirus as an active ingredient against the apple moth. The methods for producing strains of the Cydia pomonella granulovirus to develop bioinsecticides are explained. The methods for the reproduction of laboratory populations of insects, the method of their infection and isolation of viral onset are described. The stages of obtaining bioinsecticides in vitro, as well as methods for their storage are provided
Genetic studies of apricot are the actual direction in the genetics of fruit crops. In this regard, the improvement of the collection of SSR markers for the genotyping of this culture is an objectively significant task. In a study for the 16 SSR-markers previously developed on almonds (PdUnchar2, PdSLD1, PdGMGT1, PdTrTFGT1, PdUnchar2, PdSLD1, PdGMGT1, PdTrTFGT1) and Siberian apricot (A3-72, A1-63, H2-22, A3- 7-1, H2-5, A1-7, A3-9, H2-45), approbation and evaluation of the prospects of using for genotyping Prunus armeniaca L. were performed. Approbation, performed on 3 varieties of different origin, revealed markers and their combinations optimal for their use. During the study, all tested DNA markers were grouped into multiplex sets, including 4 markers. This allows carrying out genotyping simultaneously on 4 loci in the formulation of one reaction. One marker (PdUnchar2) from the studied sample included in the multiplex set did not show amplification. Five markers gave a monomorphic product. The remaining 11 SSR markers allowed us to obtain polymorphic, cultivar-specific SSR fingerprints for all the studied cultivar. These multiplex sets are proposed for use in studying the genetic polymorphism of the species Prunus armeniaca L.
This work is a continuation of the author's series of works on cognitive veterinary medicine. The present period is characterized by the appearance of huge volumes of texts in different languages in the open access, generated by people. Currently, these texts are accumulated in various electronic libraries and bibliographic databases (WoS, Scopus, RSCI, etc.), as well as on the Internet on various sites. All these texts have specific authors, dates and can belong simultaneously to many non-alternative categories and genres, in particular: educational; scientific; artistic; political; news; chats; forums and many others. The solution of the generalized problem of attribution of texts is of great scientific and practical interest, i.e. studying these texts, which would reveal their probable authors, date of creation, the ownership of these texts to the above generalized categories or genres, and might evaluate the similarities - differences of authors and texts according to their content, highlight key words etc. To solve all these problems it seems necessary to form the generalized linguistic images of texts into groups (classes), i.e. to form semantic kernels of classes. A special case of this problem is the creation of the semantic kernel in various scientific specialties of the HAC of the Russian Federation and the automatic classification of scientific texts in the areas of science. Traditionally, this task is solved by dissertation councils, i.e. experts, on the basis of expert assessments, i.e. in an informal way, on the basis of experience, intuition and professional competence. However, the traditional approach has a number of serious drawbacks that impose significant limitations on the quality and volume of analysis. Currently, there are all grounds to consider these restrictions as unacceptable, because they can be overcome. Thus, there is a problem, the solutions of which are the subject of consideration in this article. Therefore, the efforts of researchers and developers to overcome them are relevant. Therefore, the aim of the work is to develop an automated technology (method and tools), as well as methods of their application for the formation of the semantic core of veterinary medicine by automated system-cognitive analysis of passports of scientific specialties of the HAC of the Russian Federation and automatic classification of texts in the areas of science. A detailed numerical example of solving the problem on real data has been given as well