Name
Koshchayev Andrey Georgiyevich
Scholastic degree
•
Academic rank
professor
Honorary rank
—
Organization, job position
Kuban State Agrarian University
Web site url
—
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Articles count: 41
The article reflects information characterizing the significance of mastitis and endometritis in the indicator of premature disposal of cows from herds of large farms in the western regions of the Krasnodar region: an average of 30% of the total culling of cows. The effectiveness of veterinary measures in Pravoberezhny JSC of the Temryuk district for mastitis and endometritis in cows was assessed: 81-95%; fertilization index value ‒ 1.8; the duration of the service period exceeds the requirements by 20 days. The studies the nature of the influence of the drugs called Sepranol and Uteroton on the calving process and the occurrence of postpartum complications in cows from the herd, which was formed with violation of breeding principles. The use of the drugs Uteroton and Sepranol contributed to a 50% decrease in the calving index in calving cows by 50% compared with the results of the combined use of estrophan and oxytocin. The use of Uteroton reduced the damage from the lack of milk by 87880 rubles, and the use of Sepranol - by 171164 rubles. We have given practical recommendation on the use of Uteroton and Sepranol for veterinary effects on the body of cows in the early postpartum period in order to reduce the occurrence of complications
The article demonstrates the results of therapeutic and preventive effectiveness of the use of lactic acid bacteria – Lactobacillus brevis and Lactobacillus parabuchneri in laboratory animals, in particular mice and rats. They were isolated from the gastrointestinal tract of a wild quail in experimental violation of the intestinal microbial background. Artificial imbalance of the intestinal microbiota of experimental animals was caused by the use of the antibiotic. As a result of tests it was established that the application of probiotic cultures improved microbial disorders of the gut, however, the use of their consortium in the probiotic promoted consistent clinical result characterized by an elevated level of representatives of the normal flora (lacto- and bifidobacteria) and reduction of the number of representatives of pathogenic and conditionally pathogenic microbiota, with subsequent achievement of a level characteristic of healthy animals
In the Krasnodar region there is a unique gene pool of animals both for dairy and meat. To assess the heifers the authors formed five experimental groups of animals, the most common species in the region. The authors studied the main economic, biological and exterior features: live weight, measurements, assessment of the exterior. The studies found that among intensive species with the largest body weight throughout the growing period, in 8-month old Charolais heifers superior Simmental ones at 17.4 kg. In one year old Simmental heifers were 9.2 kg (R˂0,05) ahead of Charolais and in 15 months – 10 kg (R˂0,05). Over the entire period of growth the highest rates of relative growth rates of heifers were obtained by red steppe breed type named Kuban, which had an advantage over the Aberdeen-Angus – 19.1%, Charolais – 5.6%, Hereford – 6.2%, and the difference between them and Simmental weren’t fixed. When growing, the animals become less tall, more stretched, wide and massive. We have noted that during the period of cultivation we had increased indices: stretch (9% – Red Steppe, 13.8% – Charolais, 16.1% – Hereford); chest (5.4% Aberdeen Angus). This reduces the index of long legs of red steppe – 5.6%, 10.9% for Charolais. The study of linear growth of animals confirms that the animal meat breeds of live weight increase was due to the extension of the body and increase the width of the pelvis and chest. Some different facts were observed in heifers of Red Steppe breed. For them, the most characteristic increase in the width of the chest was 1.44 times, as well as increasing the depth of the chest and chest girth behind the shoulders
The work was carried out in the scientific-research
laboratory of the chair of Biotechnology, Biochemistry
and Biophysics of Kuban SAU, the aim of which is the
search of optimal conditions of obtaining the
hydrolyzates of lactic acid bacteria by thermoacid and
enzymatic ways. The pure cultures of microflora of
gastrointestinal tract of quails − Lactobacillus agilis,
Lactobacillus intermedius and Lactobacillus salivarius
were the object of researches. Under conducting the
thermal acid hydrolysis there were used the same
correlations of bacterial mass and water as well as
procedures of clearing of hydrolyzates. The variable
conditions were рН, temperature and time of hydrolysis.
When carrying out the enzymatic hydrolysis in this
study there was used the enzyme from the group of
muramidase lysozyme. The amount of enzymes and
time of carried out hydrolysis were the variable
conditions under obtaining the enzymatic
hydrolyzates. In the process of researches there were
studied: qualitative content of total protein, peptides
with М.м. < 1500, protein and peptides with M.m. >
1500, aminoacids and as well as GMDP
(glucosaminilmuramilpentapeptide). On the basis of
carried out experiments there was stated that according
to all components of biologically active substances the
thermal acid way is more effective than enzymatic. The maximum accumulation of BAA in hydrolyzates
by different ways was revealed at destruction of cells
Lb. intermedius. Content of GMDP in thermal acid
hydrolyzate was higher than in enzymatic on
0,028 g/100 ml (51,8 %)
During the research, we have developed a low-temperature zonal heating panels with an infrared emitter to create a local microclimate for piglets in the suckling period. This heater is lightweight and durable frame in the form of an inverted box size 400 mm x 1800 mm, attached to the wall of the building swivel. Top frame mounted thermostat is connected to a temperature sensor mounted directly on a metal plate with a thickness of 1 mm, painted in black color. In the upper part of the inner carcass layer is a light insulating material, which is located directly under the low-temperature heat source is electric. Study of the optimal size of the heating panel revealed that in view of the limitations associated with the area of the machine and biological characteristics of the optimum size of the sow for 11 piglets are: width – 400 mm; length – 1500 mm (at ventral position) and 1800 mm (lateral position). Comparison of different heating sources for piglets revealed that the use of infrared lamps enables newborn piglets seating area by 55.6%, while in 28 days – by 88.9%. Infrared lamps can not completely meet the recommended specifications as to ensure a more intense heat lamp to be lowered towards the floor surface, which leads to a reduction in the useful area heated. Using as a source of local heating mats and low-temperature panels satisfies pigs in the area of the den in the early suckling period at 138.9 and 200.0%, to weaning, respectively – by 48.5 and 65.5%. Thus, we have developed a low-temperature heating panels contributed to a more uniform distribution of heat in a recreation area of suckling piglets and to provide a comfortable environment at temperatures of 2-3 C lower in comparison with other methods of heating
In the review, the stages of designing therapeutic cocktails of T4 type bacteriophages based on works by Harald Brüssow from Nestlé S.A. (Switzerland) are considered. The main stages of this process are identified: analysis of existing cocktails, selection of phages; creating a collection; cultivation of the host bacterium, multiplication of viruses; purification of the preparation; contamination testing; preservation, stabilization and storage; preclinical and clinical trials. H. Brüssow first studied the Russian drug " Coli-Proteus bacteriophage " of "Microgen" with the help of metagenomic analysis, electron microscopy and conducted its clinical studies. Prof. Brüssow considered the advantages of T4 bacteriophages for the treatment of Escherichia coli infections. Researchers studied methods of cultivation in Erlenmeyer flasks, in a bioreactor, in disposable sack cultivators for the propagation of viruses. For its purification the chromatography, centrifugation, filtration and polyethylene glycol precipitation were studied. To quickly check the contamination of phage cocktails, a mass spectrometry method is proposed. Researchers considered basic strategies, such as lyophilization, spray drying, the formation of microcrystals and microspheres to stabilize the preparations. They also reviewed the results of clinical trials of phage cocktails. We have listed the problems of selecting T4 bacteriophages from the point of view of modern knowledge. H. Brüssow and his colleagues carried out an interesting work on the construction of phage cocktails based on T4 type bacteriophages, and also revealed the problems of the current state of phage therapy
We have studied biochemical changes in the body of
cattle, sick with nodular dermatitis in the initial stage
of the clinical manifestation of the disease. It is
proved that in case of disease in animals there is a
significant increase in the level of creatinine up to
295.5 mg%. The excess of the norm by 82.1%. In
treatment, this indicator was significantly reduced (189.2 mg%), but did not reach the upper limits of the
norm (162.4 mg%). In the blood serum of patients, the
carotene content was reduced to 0.09 mg%, which
was restored to normal three days after the start of
treatment (1.0 mg%). The glucose level at the
beginning of the disease was 18% below normal and
reached the lower limit of the norm 8 hours after the
start of treatment. When studying the activity of CKK,
it was found that the activity of the enzyme in animals
before the introduction of a 5% solution of sodium
hydrogencarbonate is 82% higher than the upper limit
of the norm. After the treatment, this indicator
returned to normal. The inorganic phosphorus index
did not reach the physiological norm (2.5 mmol / l)
before and after treatment, and remained 11.6%
higher (2.9 mmol / l), which indicates a violation of
mineral metabolism in sick animals. The level of
reserve alkalinity began to recover after 8 hours of
observation and reached a minimum limit of 48.91
vol. % CO2. During the entire period of the
experimental studies, before and after the
administration of the drug, there were no significant
changes in the biochemical parameters of total
protein, calcium, vitamin E and urea. The nature of
the established changes varied within the limits of
physiological norms. The use of a 5% solution of
sodium hydrogen carbonate in nodular dermatitis
increases the effectiveness of symptomatic treatment,
reduces the likelihood of complications and stimulates
the production of specific antibodies against viruses
and bacteria. We have recommended intravenous
injection of 5% sodium hydrogencarbonate solution to
sick animals at the rate of 1 ml per 1 kg of live weight
of the animal three times with an interval of 3 days
This article provides an overview of the need of the use of probiotic supplements and medications in the diets of insects, in particular bees and their families. We present a list of both Russian and foreign probiotics and their complexes, which are used in beekeeping for the treatment and prevention of various diseases, are shown. Their effectiveness is shown not only for the prevention and treatment of microbial disorders in the gastrointestinal tract of bees due to the manifestation of antagonistic properties in relation to opportunistic and pathogenic microflora, but also the possibility of their use as tools that can increase the immune status, the safety of bee families, and life expectancy. The article shows data on the positive effect of probiotics on the physiological and biochemical parameters of honeybees, activation of metabolic processes in the formation of a fat body, growth of strength and weight of larvae, development of pharyngeal glands, etc.
A method of obtaining insertion mutants for the hoc
gene, which encodes for the main phage antigen, was
developed on the model of bacteriophage T4. This gene
was cloned in the plasmid pBSL0+ and was disrupted
by insertion of foreign DNA. The phage mutants were
obtained by in vivo phage-plasmid recombination. The
construction of insertion bacteriophage mutants was
carried out in two stages. The resulting mutants on this
procedure could be grown on wild-type E. coli strains,
which is convenient for the production and use of these
phages in therapy. The mutants obtained had reduced
antigenicity. At the same time, the yield of the mutant
strains was high when they were grown on the
non-suppressor E. coli laboratory strains. A number of
stages of purification of the bacteriophage mutants
obtained were performed. Preparations have been
studied by transmission electron microscopy and mass
spectrometry. By several periodic cultivations of the
mutant bacteriophages, it was shown that mutations of
this type are stably maintained during more than 50
generations. T4 related bacteriophages of the family
Myoviridae, for example, T-even, have the significant
homology amongst their genomes, which makes
possible to produce similar mutants. Thus, our method
was developed to obtain mutants with reduced
antigenicity which can be used for both the treatment of
systemic infections, and diarrhea in the case, when,
bacteriophages penetrate into the bloodstream. Such
phages can be used in medicine and veterinary. The
reported study was partially supported by RFBR,
research projects No. 13-04-00991, 16-44-230855
The article provides a convincing argument for the preference of wool and fur products over artificial and synthetic textile fibers. It is noted that artificial fibers and synthetics have negative ionization, while natural wool has positive ionization. It is emphasized that negative ionization is far from harmless to humans. The analysis of the state of sheep breeding in the Russian Federation is given and this industry in the Rostov region is characterized against this background. The data on the number and productivity of sheep of the main age and sex groups, the production of young animals per one hundred ewes are presented. The characteristic of tribal resources of the country and region is given. The breeding base of the industry in the Russian Federation is represented by 205 breeding organizations, including 3 breeding and genetic centers, 44 breeding plants, 145 breeding reproducers and 13 gene pool farms, which contain 1389.8 thousand sheep (39.0 % of their total number in agricultural organizations and 6.6 % of the sheep population in farms of all categories), including 904.7 thousand Queens (35.0 and 6.2%, respectively). The breeding base of the Rostov region has eleven farms, of which 6 breeding plants for such breeds as the Soviet Merino and salskaya and 5 breeding reproducers. In addition to these breed resources, there are breeding reproducers for the qigai and edilbaev breeds in the region. Total number of breeding sheep in the region is almost 44 thousand. The article indicates main problems in the development of the industry and ways and means to solve them