Two methods were used for DNA extraction from herbarium leaves of two subspecies: V. vinifera subsp. sativa D.C. и Vitis vinifera ssp. silvestris Gmel. DNA extraction is difficult from herbarium leaves plants because of the presence of metabolites that interfere with DNA isolation procedures and downstream applications such as DNA restriction, amplification, and cloning. The quality of DNA was checked on a spectrophotometer by measuring the level of absorption at A260/280 nm. The results showed the successful extraction from mature and dried leaves of DNA when using the СТАB-method №2. Another method hasn’t shown satisfactory results in amplification of DNA
Necessity of application of Emistim preparation at various stages of clone micro reproduction is investigated and proved: induction of meristems into culture, micro reproduction itself, education of roots and micro grafting
The article contains data of reacclimatization of mar-mots (Marmota bobak Muller) in Lipetsk region. Pos-sibility of application of artificial holes for fixation of colonies of animals in certain terrain is considered. Influence of a grazing of the shallow cattle on locali-ties of marmots is investigated. Degree of success of measures for reacclimatization of marmot badgers is defined
In the given and subsequent articles of this scientific electronic magazine scientific works, written by employees of chair of wine growing of Kuban State Agrarian University (in general) , executed for last years, after seven-years-old "Perfection of assortment, manufacture of planting material and grapes: the Collection of scientific articles / КSAU. - Issue 394 (422). - Krasnodar, 2002. - 186 pages" collective results of NIR will be observed
The changing dynamics of nutrients in soil, doses of mineral fertilizers, efficiency, a mode of onion irrigation on irrigated soils of Rostov region are resulted in the article
Indexes of biometric parameters of tree crowns and fruit productivity of Krupnoplodnaya, Valery Tchkalov, Vasilisa varieties depending on schemes of tree planting are presented in this article
The article presents the results of studies on the species composition of fungi isolated from seedlings of grapes affected by vascular necrosis
In the article the historical information about a condition of wine-growing and wine making branches of the Russian Federation - the most profitable branch of agriculture - from 1984 for 2008 is resulted; statistical and economic indicators are stated. For example, the area of vineyards has decreased from 190,1 thousand in hectares to 64,4, productivity fluctuated within 30,1-79,5 centner/hectares, etc. The information about wine-producing subjects of the Russian Federation Is resulted. The Program of development of wine growing and winemaking of the Russian Federation for 2008-2020 is observed, according to which the increase in the areas of vineyards from 64 to 172 thousand in hectares is provided, increase in volumes of manufacture of grapes from 300 thousand to 1,1 million tons, manufacture of table grapes will increase in 2 times, juice to 100 thousand gallons; it will be employed about 200 thousand persons in addition; the economy of regions will increase more than in 3 times is covered
In this article the perspective of using Lignas and Lavarin microfertilizers, which increase milt of winter wheat and give economical effect is shown
The article reflects the results of experimental studies in heterosis breeding of corn. We defined the common (CCA) and specific (SCA) combining ability of the new material. We also obtained the valuable inbreeding lines and testers of corn, created based on these new hybrids. The final step was the evaluation of hybrids and calculation of economic efficiency of their implementation. As a result of the work, it was allocated the three hybrid combinations of corn and suggested to examine them in strain testing