Scientific Journal of KubSAU

Polythematic online scientific journal
of Kuban State Agrarian University
ISSN 1990-4665
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368 kb

PROGNOSTIC SIGNIFICANCE OF LYMPHOCYTIC INFILTRATION IN VULVAR CANCER PATIENTS

abstract 1251701056 issue 125 pp. 827 – 839 31.01.2017 ru 8
A retrospective immunohistochemical study of lymphocyte and macrophage infiltration in tumor tissue of 84 vulvar cancer patients with a long (over 2 years) and short (less than 2 years) remission was performed to determine a possible role of immunologic microenvironment of tumors in maintaining remission. Vulvar cancer patients with a short remission period were aged 46-85 years, mean age 67 years. 15.8% of them had stage I of the disease, 21% - stage II, 47.4% - stage III and 15.8% - stage IV. The average duration of remission in this group was 11 months. Patients with a long remission period were aged 27-71 years, mean age 48 years. 38.5% of them had stage I of the disease, 53.8% - stage II and 7.7% - stage III. The average duration of remission in this group was 8 years. Histological study showed squamous cell carcinoma in all patients. Stroma and parenchyma of tumor tissues demonstrated lymphocyte and macrophage infiltration of various degrees with the predominance of T-lymphocytes. CD8+ cells prevailed in tumor tissue of patients with a long remission period (over 2 years), while a higher level of CD4+ Т-lymphocytic infiltration was associated with a short remission (less than 2 years) and a poor prognosis. These differences allow prediction of the vulvar cancer course and its early or late recurrence
131 kb

CLINICAL AND MORPHOLOGICAL FACTORS AND PREDICTION PARAMETERS OF CELL CYCLE IN ESOPHAGEAL CANCER

abstract 1241610028 issue 124 pp. 484 – 493 30.12.2016 ru 36
The aim of the study is to compare the traditional clinicopathologic factors and prognosis of cell cycle parameters, such as ploidy, cell cycle kinetics, as well as the study of DNA content in the tumor tissue of patients with esophageal cancer. Cancer of the esophagus - a very aggressive malignant disease, most commonly manifested impaired swallowing. As a rule, this type of cancer pathology is characterized by unfavorable outcome. Ploidy and cell proliferation activity, defined by the content of the DNA of the tumor - synthesizing cells are one of the important indicators of the growth rate of malignancy and prognosis. The study was performed on tumor tissue fragments of the 30 patients operated on for cancer of the esophagus. DNA study - conducted in the open cell material aftercare. DNA cell content was determined by flow cytometry BD FACSCanto II. By studying the DNA-cytometric parameters, we found the prevalence of aneuploid tumors in 66.7%. It was shown that DNAcytometric parameters are independent of gender. Major weight of diploid and aneuploid cell tumors of the esophagus was in G0 / 1 phase of the cell cycle. Comparative analysis of DNA - cytometric parameters and the degree of differentiation of esophageal tumor revealed a prevalence of aneuploid tumors in all the test cases. content distribution pattern of tumor cells was found in the G0 / G1-, G2 + M -, S - phase of the cell cycle at different stages of the disease
299 kb

NON-INVASIVE DIAGNOSTICS OF ANEUPLOIDY OF A FETUS WITH CIRCULATING TROPHOBLAST CELLS IN MATERNAL BLOOD

abstract 1231609131 issue 123 pp. 1965 – 1984 30.11.2016 ru 71
Isolation of fetal cells from the blood of a pregnant woman is more relevant for receiving the information of the genome of a fetus and implementation in clinical practice, non-invasive screening methods for prenatal diagnosis. The work proposes efficient ways of sorting trophoblasts circulating in maternal blood for the diagnosis of fetal aneuploidy in early gestation by analyzing microsatellite marker of the chromosomes that most prone to numerical aberrations (13, 18, 21, X and Y). A set of microsatellite markers allowed to reveal aneuploidy in three pregnant women, and also to exclude mosaicism and contamination of samples with maternal cells. A retrospective analysis of data on invasive cytogenetic studies (chorionic villus sampling) confirmed the results of the study
165 kb

HIFU: PROSPECTS OF APPLICATION IN ONCOUROLOGY

abstract 1221608019 issue 122 pp. 264 – 277 31.10.2016 ru 108
Prostate cancer (PC) is one of the most frequently malignant neoplasms in the worldwide male population. Back in the 90's began a promising noninvasive method of high-intensity focused ultrasound (HIFU) as an alternative to radical prostatectomy for the treatment of localized prostate cancer was used. Information about the destructive histological effect by HIFU has been known since the 1930, however, the clinical implementation of this technology due to the absence at the time visualization capabilities for monitoring during the procedure has been postponed. In this paper, an overview of published data on the HIFU clinical application in prostate cancer as a major, "saving" and focal therapy. Examples of a favorable combination of this method are also provided with pharmacological agents. Based on the analysis of clinical data, we can say that HIFU is a good alternative minimally invasive therapeutic modality for patients with prostate cancer who are not candidates for radical prostatectomy. Applying of HIFU is possible as a treatment option with local recurrence after external radiotherapy, with careful selection of patients is important, depending on prognostic factors
141 kb

THE COMPARATIVE CHARACTERISTICS OF CTCs IN COLORECTAL CANCER PATIENTS DEPENDING ON THE CHOICE OF SURGICAL INTERVENTION

abstract 1221608073 issue 122 pp. 1064 – 1074 31.10.2016 ru 104
Mortality from malignant diseases is directly linked to early metastasis and relapse. It is now possible to identify individual tumor cells in bone marrow biopsies and peripheral blood. Colorectal cancer is one of the leading places in the structure of cancer incidence and mortality, both in Russia and around the world. Studies have shown that after surgery the ability of tumor cells to the dissemination and implantation increases. The work contains 30 patients with the verified colorectal cancer with metastasis to the liver. To determine the CSC we have applied an analysis technology in CellSearch System ™ (Janssen Diagnostics, LLC). Our study of the number and frequency of detection of circulating tumor cells in peripheral blood of patients and control group demonstrates the reduction in the number of CTCs during laparoscopic surgery and changing the dynamics of the level of tumor cells in the form of reduction from the stage of the mobilization of the drug due to minimally invasive access, minimizing manipulation in the projection of the tumor and as a consequence, preventing the release into the blood stream of single tumor cells
176 kb

ROLE OF PLASMAPHERESIS AND XENON THERAPY IN CORRECTING THE ACUTE EFFECTS OF SURGICAL MENOPAUSE IN PATIENTS WITH CERVICAL CANCER

abstract 1171603029 issue 117 pp. 475 – 489 31.03.2016 ru 303
The article analyzes the effectiveness of plasmapheresis and xenon therapy in 40 patients with gynecological cancer who underwent surgery in the amount of extended panhysterectomy about cervical cancer, who developed signs of post-castration syndrome in the immediate postoperative period. The content of gonadotropic pituitary hormones was determined in the blood of patients with cervical cancer and healthy women - follicule-stimulating, luteinizing hormones, prolactin; steroid hormones: estradiol, testosterone, cortisol; thyroid hormones. It is found that xenon therapy has a more pronounced antistress, hormone-modulating, sedative and antidepressant effect and is more preferable to treat post-castration syndrome. Performing of plasmapheresis in this aspect is most justified in patients with concomitant metabolic syndrome
129 kb

VAGINAL MICROECOLOGY IN WOMEN WITH METABOLIC SYNDROME IN CLIMACTERIC PERIOD

abstract 1171603054 issue 117 pp. 833 – 840 31.03.2016 ru 276
The aim of the research is to study the peculiarities of vaginal microbiocenosis in female patients with metabolic syndrome during peri- and postmenopause. 320 women in the climacteric period aged of 45-70 were examined. Two groups were formed: the main group (160 female patients with metabolic syndrome), the control group (160 women without metabolic syndrome). The average age of women 54.5 ± 7.2 years. Depending on the length of menopause each of the two groups was divided into three subgroups.Microscopy of vaginal smears, the colpocytological analysis, the culture test of vaginal discharge were conducted. The statistical processing of the results was carried out with the help of programme sets Microsoft Office 2010 (MicrosoftExcel) and «STATISTICA® for Windows 6.0». A tendency of decreasing frequency of non-specific vaginitis with aging and of increasing frequency of vaginal atrophy, disorders of vaginal biocenosis (lactobacillus deficiency and the domination of conditionally pathogenic microorganisms) was discovered. The frequency of vaginal atrophy in women with a length of postmenopause of 10 years and older was 65.9% in the subgroup of in female patients with metabolic syndrome and 63.6% in the subgroup of female patients patients without it. The more observable changes in vaginal biotope were found in women with metabolic syndrome
160 kb

THE POSSIBILITIES OF NON-DRUG CORRECTION OF UROGENITAL DISORDERS IN FEMALE PATIENTS WITH THE METABOLIC SYNDROME DURING THE MENOPAUSE

abstract 1161602003 issue 116 pp. 70 – 81 29.02.2016 ru 481
The aim of the research was to assess the effect of nondrug correction of urogenital disorders in female patients with metabolic syndrome during the menopause. The research includes 330 women aged 45-50 with metabolic syndrome. Three equal groups with 110 female patients each were formed: the main group, the first control group, and the second control group. In the main group, the non-drug treatment (diet therapy, psychotherapy, physical therapy, exercise therapy) and the vitamin-mineral treatment were combined. In the first control group, only non-drug treatment was used. In the second control group, only the vitamin-mineral treatment was used. Before the treatment and in six months after the treatment, the following was assessed: the intensity of vaginal atrophy symptoms according to the 5-point Barlow scale; Bochman vaginal health index; urinary diaries data. The results of the research showed: the average value of Bochmanvaginal health index has most significantly increased in the main group (from 3,64±0,63 pointsto 4,67±0,68 points, p
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THE EFFECT OF NON-DRUG TREATEMENT OF CLIMACTERIC DISORDERS ON THE GASTROINTESTINAL TRACT IN FEMALE PATIENTS WITH METABOLIC SYNDROME

abstract 1161602104 issue 116 pp. 1661 – 1670 29.02.2016 ru 0
The aim of the research was to assess the effect of nondrug correction methods of climacteric disorders on the digestive system in patients with metabolic syndrome. 330 women aged 45-50 with the diagnosed metabolic syndrome were examined. Three comparable groups were formed with 110 female patients in each group. In the main group the methods of the nondrug treatment (diet therapy, psychotherapy, physical therapy, exercise therapy) and the vitamin-mineral treatment were combined. In the first control group only the non-drug treatment was used. In the second control group only the vitamin-mineral treatment was used. The female patients’ complaints and the biochemical profile parameters were assessed before the treatment and six months after the treatment. Ultrasound scanning of the abdominal cavity, fibrogastroduodenoscopy and videocolonoscopy were carried out. The results of the study are as follows. In all patients the digestive tract problems were diagnosed at the beginning of the research. Moreover, combined damages were noticedin most of the patients. The main group demonstrated the most pronounced positive dynamic according to the subjective evaluation of the symptoms as to the gastrointestinal tract, as well as according to the parameters of the carbohydrate and fat metabolism. We may conclude that the complex non-drug correction of climacteric disorders in female patients with metabolic syndrome, directed on the increase of the adaptation abilities of thewomen’s organism, the normalization of all kinds of metabolism and of the endocrine profile, has the most important positive effect on the organs of the gastrointestinal tract. This, in its turn, well coordinates all the kinds of metabolism
203 kb

TRANSCRIPTOMIC ACTIVITY OF ESTROGEN-REGULATORY GENES IN MALIGNANCY UTERINE TISSUES

abstract 1151601017 issue 115 pp. 294 – 304 27.01.2016 ru 374
Uterine cancer is the sixth on prevalence of cancer among women in developed countries. Hyper-estrogen level by endogenous or exogenous origin plays the leading role in this disease pathogenesis. This study purpose was investigating the expression of genetic loci responsible for the estrogen reception and metabolism to better understanding of the molecular mechanisms of uterine tissues malignancy. We investigated the relative expression of genes (CYP1A 1, CYP1A 2, CYP1B 1, CYP19A, ESR1, ESR2, GPER, STS, SULT1A and SULT1E1) by real-time PCR in tumor and conditionally healthy uterine tissues of 27 female patients in Southern Russia aged 38-72 years with histologically confirmed diagnosis of uterine cancer. It is found that transcription activity of these genes during malignancy of uterine tissues depends of female patients age and the stage of tumor cells differentiation. It is found that malignancy change in the transcriptional activity of investigated genes in uterine tissue depends of patients age and stage of tumor cells differentiation. Particularly, it was found, that changes of gene transcription balance of enzymes that provide sulfation and hydrolysis of steroid sulfates is depending of patient age and the degree of tumor cells differentiation. In addition, the sinking of gene expression of nuclear estrogen receptor ESR1 as lowering cell differentiation of tumor tissues. We have also shown gene expression of nuclear estrogen receptor ESR1 decline as lowering cell differentiation of tumor tissues
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