Scientific Journal of KubSAU

Progress of breeding for resistance to diseases, pests and low temperatures is provided by cross-species hybridization. Institute of Vine and Wine "Magarach" is one of the leading centers of selection of grapes in the world. The breeding program of deducing grapes at the institute is based on the study and attracting the world's gene pool, including and industrial of the Russian Federation assortment of grapes. Analysis assortment shows that the share of productive, highquality varieties with group resistance to diseases, pests and extreme environmental factors is very small. The main method of selection of grapes of a new generation who have genetically determined traits earliness, resistance to biotic and abiotic factors is crossing complex interspecific hybrids and forms of their involvement with each other and with the forms of species Vitis vinifera, belonging to different ecogeographical groups, saturating crossing between hybrid forms with complex features, the appropriate selection of reference. For vigorous offspring is most effective as a parent to use a local variety of forms of Crohn and father - interspecific cultivar Alminsky

This article presents the possibilities of using organic fertilizers, which allow you to recharge grape raw material with biologically active substances and improve the nutritional value of wine production. The influence of organic fertilizers on activation of process for detoxification of soil toxic compounds has been measured, ensuring food safety of raw materials for the production of grape wine products by reducing of hazardous chemicals. The use of biotechnology preserves and also enhances the uniqueness and diversity of the chemical composition of the grape raw material. We have established that after the four-year application of biotechnology the content of toxic compounds in grape raw material was reduced by 7%, and the concentration of biologically active substances increased in 1,5-2% times in comparison with the production of commercial plantations

The article presents the structural and phylogenetic analysis of VIT_02s0033g00410, VIT_02s0033g00390 and VIT_02s0033g00450 genes of the grapevine genome and the closely related orthologous gene MYB114 of the Arabidopsis genome. These genes are responsible for the biosynthesis of anthocyanin in the organs of model plants and are of interest not only for practical production and breeding, but also for fundamental research. These genes were analyzed for GCcomposition of nucleotides, the presence of cisregulatory elements and promoter regions. DNA and protein sequences were aligned to look for similar elements, which allowed further analysis of the ultraconservative domains of four genes. Based on the results of search and identification of the conservative regions, a cluster tree was constructed, which made it possible to identify the separation of gene sidelines from, presumably, the main one. At the same time, the construction of consensus trees based on DNA and protein sequences revealed their absolute similarity. "The Minimal Evolution Tree" allowed calculating the approximate dates of the appearance of the mutations and the divergence times of the gene branches between each other. At the same time, the appearance of the Vitis genus and its separation from the Rosales was taken as the time first divergence point. In the end, homologous metabolic pathways were searched between grapevine and Arabidopsis, which revealed the presence of homologous proteins in the grape proteome. In this turn, it already confirms the existence of similar biosynthetic pathways and, as a consequence, interactions such as "DNA-protein" and "protein-protein"

In January 2015, at the Vineyard and ZAO "Pobeda" in Temryuk District of the Krasnodar region, the thermometer dropped to minus 21,6 C, which resulted in significant damage to the central wintering buds. The article presents a method of determining the degree of damage of wintering buds. Based on the results of inspection of variety by sections divided into 3 groups. Group 1: saved between 40 and 72% central. This group includes the following 7 varieties : Pervenets Magaracha (uch. 64) Gibernal (uch. 74/3), Viorica (uch. 77), Riton (uch. 66), Riesling (uch. 82), Traminer (uch. 73/2), Chardonnay (uch. 73/3). Cutting of bushes should be carried out by the usual way. The task of cutting - compensate the loss of crops due to leaving the greater load. Group 2: Central buds was damaged nearly 70-85%, saved from 20 to 35% of replacement buds. This group includes Merlot (uch. 83), Tsitronyy Magaracha (uch. 69), Augustine (uch. 66), Saperavi (uch. 79), Chardonnay (uch. 74/1), Muscat white (uch. 73) Cabernet Sauvignon (uch. 80). During cutting should be left as much as possible of annual shoots and well-developed secondary shoots. Group 3: damaged more than 85% of central buds, replacement buds significantly damaged . To this group assigned Saperavi (uch. 80), Pinot blanc (uch. 74), Merlot (uch. 80/2), Moldova (uch. 70), Chardonnay (uch. 68), Moldova (uch. 22) Kaberne- Sauvignon (uch. 68), Moldova (uch. 38), Arkadia (uch. 78), Tsimlyansky black (uch. 68). Pre-cutting consists in removing all clearly dead and unusable parts of the bush (dried vines, stumps, annual shoots) that will accelerate the development of shoots from the angular buds and resting buds. Considering the high plasticity of grape plants, can not only in accelerated way to restore the crown of bushes damaged by frost, but also get a crop in the current year, using special methods of agricultural technology

The article presents the characteristics of the mechanical composition of clusters, the chemical composition of grape juice and fresh grapes and wine tasting evaluation of three varieties of table. We have identified the following uvologic indicators of clusters of cultivars Flora, Nisina and Anyuta: structure - 90.0; 91.5; 87.3; addition - 8.0; 6.6; 10.8; structural - 6.4; 5.4; 8.1; berries - 17.6; 15.0; 10.4. In the studied berries of grape varieties the mass concentrations of sugars and titratable acids were in the range 18,1-23,5 and 0,73-0,78 g/100 cm3 respectively. The combination of sweetness and acidity causes high taste properties of grapes. In grape berries of Flora and Nizina the concentration of fructose was two times more than glucose. Differences in the content wine, malic, lemon and succinic acids in berries varieties of Flora and Nizina were insignificant. In the berries of Anyuta variety, the concentration of lemon and succinic acids was more, malic was less than other varieties. Most of the studied varieties of berries contain malic and wine acids. More of cations of potassium, sodium, calcium and magnesium then in other varieties and it contain less than Anyuta berries. The difference in the content of various cations in the berries of varieties of Flora and Nizina is negligible. Tasting score of fresh grape of Flora variety was 7.0, Nisina - 7.8 and Aniyta - 7.6 points

The article contains the results of the study of mechanical composition of grapes and biochemical characteristics of white wine grapes for the production of juice of direct extraction and blending. Thus, the yield of studied grapes ranged from 7.5 to 13.2 t / ha: highest yielding varieties and hybrids of Pervenets Magaracha and Tsitronnyy Magaracha, and the lowest - Chardonnay and Pinot blanc. At an average weight of clusters of varieties were divided into three groups - large clusters (230-243 g) in varieties Pervenets Magaracha and Tsitronnyy Magaracha, medium (181-184 g) - the varieties Chardonnay and Pinot blanc, small (112-116 g) - the varieties Bianca and Viorica. By weight of the 100 berries were divided into four groups - Bianca and Viorica (I), Pervenets Magaracha (II), Chardonnay (III), Pinot blanc and Tsitronnyy Magaracha (IV). Weight of 100 seeds from the largest varieties Pinot blanc and Bianca, and the lowest - in the varieties of Tsitronnyy Magaracha and Chardonnay. The solids content in the juices of these varieties ranged from 19.4 to 21.8%, which corresponded to branded products and higher of commodity varieties. Mass concentration of sugars ranged from 18.4 to 21.3 g / 100 cm3. Titratable acid content in the juice was varied from 0.65 to 0.78 g / 100 cm3. Sugar-acid ratio, defined as the ratio of concentration of sugars and titratable acids ranged from 23.6 to 30. The recommended values for this indicator were within 22-30. According to the results of the tasting, we rated "excellent" the juices from the varieties of Tsitronnyy Magaracha, Viorica (19 points) and Bianca (17 points); "good" marks were given to the juices from Pinot blanc, Chardonnay, Pervenets Magaracha: this means that we can get juices of excellent quality from the grape varieties under study with the title of ampelographic variety; in addition, the juice of the grape of varieties Chardonnay, Pinot blanc, Bianca, Pervenets Magaracha due to their potassium content will be functional to improve the cardiovascular system. On the basis of the tasting we have conducted mixing of the juices from the varieties of Viorica and citron Magaracha with other juice varieties 50:50 and 70:30. Sensory evaluation showed that blending juices from the varieties of Viorika and citron juice Tsitronnyy Magaracha other varieties improves product quality and 50:50 increases by one the total score, and 70:30 - two units. Blend of the varieties of Viorica and Tsitronnyy Magaracha does not increase the total score, but gives the product a special flavor and aroma. Hence, using the grape varieties under study we can produce varietal juices with the name of their ampelographic type and blended juices to extend the assortment

The article contains the results the study of the mechanical composition of clusters and biochemical characteristics of the red wine grape varieties of Cabernet Sauvignon, Cabernet Franc and Merlot (regionalized), Cabernet Carbon, Cabernet Kortis and Verdot black (introduced). In terms of the structure of the cluster of the variety they can be divided into three groups: Cabernet Kortis and Cabernet Sauvignon (16,6-16,8), Cabernet Carbon, Cabernet Franc and Verdot Black (17,5-17,9), Merlot (19.9 ). In terms of addition of the variety they can be divided into four groups: Cabernet Franc (6.3), Cabernet Sauvignon and Merlot (4.9-5.0), Cabernet Carbon (4.3), Verdot Black and Cabernet Kortis (3, 7-3,4). Structural parameters of the clusters of the varieties we have studied were ranged from 5.1 to 5.6, and the berry from 64.3 to 74. The yield of the wort from Cabernet Sauvignon, Cabernet Franc and Merlot is equal to 75,1-77,7% from Cabernet Carbon, Cabernet Kortis and Verdot Black - 70,5-72%. Mass concentration of sugars in the studied varieties was in the range of 22,3-26 g / 100 cm3, titrated acids - 0,47-0,77 g / 100 cm3, active acidity - 3.1-3.8. The ratio of fructose to glucose was: Verdot Black - 1.2, Cabernet Sauvignon and Cabernet Carbon - 1.5, Cabernet Kortis - 1.8, Kabernet Franc - 2.8, Merlot - 3.2. Most ranged organic acid in all varieties – wine acide. Relation of the content of tartaric and wine acids in varieties amounted: Cabernet Kortis 7: 1, Cabernet Karbon 6: 1, Cabernet Franc 2.8: 1, Cabernet Sauvignon and Merlot 2: 1, Verdot Black 1,4-1. Mass concentration of cations in the varieties was (in mg / dm3): potassium - 816,4-1770, sodium - 13,03-21,31, magnesium - 76,21-106, calcium - 52,24-89,45. In comparison with Cabernet Sauvignon larger potassium cations had Cabernet Franc and Merlot, sodium cations - Cabernet Carbon, Cabernet Franc, Merlot, magnesium cations - Cabernet Carbon, Kortis, Cabernet, Merlot, calcium cations - Cabernet Kortis, Merlot and Cabernet Carbon

The article presents the results of the studies of 11 varieties cultivated in the eastern region of South Coast wine-growing zone of Crimea (planting of "Solnechnaja Dolina ", near Sudak mountain) in the area of their natural growing, where was formed the gene pool of grapes for a long time. As a result, the three-year (2010-2012) study found that the majority of indigenous varieties had small number of fruitbearing shoots in the bush: the varieties with colored berry - 53,3-73,3%, in white-berry - 46,6-75,9% except Shabash variety - 79.9%. We have identified the most productive varieties which were Dzhevat kara (62.2 t / ha), Kokur Beliy (48.9 t / ha), Kapselsky Beliy (44.4 t / ha), and varieties with female type of flower: Kefesiya, Ekim kara, Kock pandas, Sary pandas and Crona gave not high yield: 22.2; 22.2; 24.4; 28.9 and 28.9 t / ha. On the strength of growth, productivity and the accumulation of sugars in the juice of berries, we picked out varieties of Dzhevat kara, Crona, Kapselsky beliy. According to the main economic indicators, it turned out that the most profitable varieties are Dzhevat Kara, Kokur beliy and Kapselsky beliy. Hence, these varieties in conditions of the Crimea have much greater prospect of reproduction and development of production

The article describes the history of the institute called "Magarach" and the formation of the world's ampelographic collections in the Crimea and Kuban, provides data on the genetic diversity of the original parent Crimean collection of grape, carries out the impact of its long-term ampelo-breeding and genetic work. We have found the best ampelo-genetic rennets, both in the Crimea (zoned in Russia and abroad varieties of Academician Avidzba, Pervanets Magaracha, Jubilee Magarach, Antei magarachsky, Nimrang ustoichivii, Podarok Magaracha, Riesling Magaracha Novoukrainsky Rannii, Granatovii Magaracha, Krymchanin, Rkatsiteli Magaracha, Spartanets Magaracha, Tavkveri Magaracha, Citronnii Magaracha, promising varieties Livya, Solnetchnaja grozd, etc..) and in the Kuban region. Magarach ampelographic collection was and still is the parent of many national collections of gene pools of, the former Soviet republics, including the Russian Federation as well: Anapa ampelographic collection in the North Caucasus NIISiV has 3356 samples collected from 41 countries, including ones from the institute of "Magarach" (about 40%). Using its base we had a comprehensive biological and economic evaluation of the outstanding genotypes for ampelo-genetic and clonal selection – such as zoned Bogotyanovsky, Helios, Dolgogdannii, Kubattik, Libya K, Arathi, Preobragenie, Rochefort K, Chrizolit, Citrine, Anthracite, promising varieties Annie, Baikonur, Gourmet Kra, Memory Master Nesvet Gift, Jubilee Novocherkasska, etc.

New wine grape flavored pink berry grape variety named “Radost Leonidov” breed at Athens Institute of Viticulture by P.Zamanidi, L.Troshin and P.Radchevskii in 2009 by crossing the new Moskhoragos Greek muscat variety with old European variety Traminer pink. According to morphological and biological characteristics, it can be assigned to Western European eco-geographical group of varieties. Strong growth of shoots (2,1-3,0 m). The extent of maturation of vines is very high (over the entire length, except for the top). Duration of production period from bud burst to harvest - 146-155 days. Productivity is high: 20-25 t / ha. The average weight of clusters is 260. Characterized by high winter hardiness, drought resistance and increased resistance to fungal diseases in comparison with the varieties of Vitis vinifera. Flower is hermaphrodite, fully developed stamens and gynoecium. Bunch is small, conical, sometimes winged, average density. Berry is small, spherical, pinkish in color, with a thick waxy coating. Peel of medium thickness, dense and durable. Pulp and juice with a strong varietal flavor, similar to the aroma of Traminer pink. Sugar content is very high: more than 24%. Cluster of the variety of Radost Leonidov at overripening is drying and stored on the bushes for a long time. The variety is intended for the production of dry white wines of excellent class, as well as highquality sparkling wines, dessert and sweet wines; suitable for the production of high-quality aromatic juices