Effects of different levels of vitamins (thiamine, pyridoxine and nicotinic acid) and macro-elements (MS, NN and PG) on development of globular, heart- and torpedo-stage embryos and plantlets with green cotyledons and on shoot production from them were studied in grapevine interspecific hybrids: cvs. ‘Bianca’, ‘Podarok Magaracha’ and ‘Intervitis Magaracha’. Pro-embryogenic calli were derived from petiole explants on solid full-strength NN medium supplemented with different levels of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzyladenine (BA) for each cultivar and used to initiate cell suspensions in liquid NN medium supplemented with 1 mg L-1 2,4-D and 0.2 mg L-1 BA. The following formulations of media were optimal for different stages of somatic embryogenesis and for plantlets with green cotyledons development: liquid NN medium supplemented with 0.5 mg L-1 BA for globular embryo formation; liquid HTE medium (with PG macro-elements, thiamine and pyridoxine at 5 mg L-1 each and 0.5 mg L-1 nicotinic acid): supplemented with 0.2 mg L-1 BA for heart-stage embryo development, supplemented with 0.1 mg L-1 indole-3-acetic acid (IAA) and 30 mg L-1 sodium humate for torpedo-stage embryo development and supplemented with 0.5 mg L-1 gibberellic acid (GA3) for the growth of plantlets with green cotyledons. Solid MS medium modified by the addition of nicotinic acid, pyridoxine and p-aminobenzoic acid (PAB) at 5 mg L-1 each, 0.5 mg L-1 thiamine and 0.5 mg L-1 BA was best efficient for shoot production from plantlets. Inflorescence formation in regenerated plants of cv. ‘Podarok Magaracha’ grown on solid hormone-free PG medium was induced by addition of nicotinic acid, pyridoxine and PAB at 5 mg L-1 each, 0.5 mg L-1 thiamine and 0.2 mg L-1 BA into liquid modified HTE medium for growth of plantlets with green cotyledons and into solid modified MS medium for shoot production from them.
In the world, huge work is being done to create global
information banks of plant genetic resources. The need
for conservation and rational use of genetic resources
in modern conditions has become very relevant.
Genetic collections are the basis for selection work:
creating new varieties, replenishing the assortment
with new, classic, introduced and native varieties,
adapted to the natural (soil and climatic) conditions of
the cultivation sites. In the Anapa ampelographic
collection, 4911 varieties of various ecogeographical
and genetic origin are represented, the use of which
provides great opportunities for creating and breeding
new varieties, their state testing and zoning. In the
gene pool of the ampelographic collection, most
varieties of V. vinifera L. grapes are represented by
local honeycombs of different regions of the grape
culture, and more than one fourth of varieties of V.
vinifera L. are obtained from intraspecific crossings.
Despite the value of local varieties of grapes, they do
not always meet all the requirements of production.
Therefore, to improve the local assortment, it is
necessary to carry out selection - breeding new
varieties of grapes. This article presents the long-term
results of the work on the selection of varieties of table
and technical directions in the Anapa ampelographic
collection used for breeding. Also, the role of the
genetic collection in the creation of new generation
varieties by combinative selection is shown - as
donors for creating new varieties of grapes. Dedicated
varieties are the sources of valuable traits, which are given primary importance and are especially important
for the viticulture of the Russian Federation
Results of four-year (2004-2007) researches of
perspective technical grape varieties by contrast
zones of Krasnodar region : in central- Antaris,
Beisug, Cabernek, Clairette Temryk, Litdar, Merlok, Muscat Kuban, Rislinalk, Risling Kuban, Shardonek, in Anapa-Krasnostop AZOS, Rubin AZOS and to the memory of Zotkina, were cited in the article. It was shown their superiority under control varieties.
Ampelographical screening of gene pool was carried out in subzone Temryk on the plot of I.G. Karas’
in 2007 and in the result of which there were selected some perspective varieties: Arcadia, Victoria, Hybrid 342, Long-waited , Lora, Original.
On the basis of the researches aimed on improving of nutritional regime of chestnut soils in the system of field crop rotations, at the same time with traditional cultivation, it can be recommended to use direct sowing
The analysis of use of glauconitic sand is provided in the article as a potential local fertilizer in the conditions of Kuban. The research of its agrochemical properties showed high value of this substance as fertilizers. In its structure we found macro - and microcells which are necessary for mineral nutrition of plants. Fractionation of phosphorus and potassium in various acids showed their different availability for the plants, which will help to consider glauconite as a fertilizer of prolonged action
In this article we have described a study of genotyping of new three productive wine grapes protoclones: Rhine Rieslin, Verdot black and Johanniter
In this article we have described our work on genotyping of new ten productive wine grape protoclones
This article presents the results of genotyping of new
perspective table and technical varieties and grapes
protoclones. It was established that varieties of Citrine,
Helios, Arcadia pink and Preobragenie show differ
genetic diversity in four loci
Farms of Krasnodar region purchase cattle of milk, combined and meat direction of productivity. Imported cattle are accompanied with documents which differ from analogous ones specified in such cases by pedigree service of Russia. There were studied 1000 heads of symmental cattle of milk-meat direction of productivity imported from Austria. More than 5000 kg of milk can be obtained from cattle of symmental breed of milk-meat direction of productivity, except it they show enough high meat productivity. The analysis of accompanying documents on purchased cattle is joined not only with language barrier but with the lack of knowledge of adopted abbreviations, contractions and other attributes used by foreign breeders abroad. Having studied the accompanying documents connected with hereditary information of purchased cattle there was determined that imported heifers and their mothers were obtained in Austria, and mothers of bulls on 55,5% obtained in Austria and 41,6% in Germany. So, on formation of imported micropopulation of cattle greatly influenced the breeding school of Germany. In accordance with the adopted classification in Austria due to revealed cases of adoption of Holstein blood, there is a ground to consider the present herd as a belonging to the group of pure breed of symmental cattle and in prospect to conduct the mating of cattle with pure breed symmental oxen with the aim of absorption of mixtures of Holstein blood in pedigree. The researches determined that one can purchase the producers with characteristics of milk productivity at daughters of oxen not lower than Austrian indexes on voluminous milking and German – on milk quality, the purchase of symmental cattle from other countries must be subjected to this demand.
There are materials about different genetic methods of metotical and meyotic autopolyploid of grapes in the article. Main morphodiagnostical signs of autopolyploids in it are pointed. New forms and varieties of grape may easily form with the help of genomic mutation. The experimental polyploid on grape leads to functional changes, which helps to become aliveness and stable in bad environment. The increasing of such kind of main genetic signs in grape, as high stable to bad ecological facts, illness and insects, high growing, seedless, high productive from bush, becoming sugar gather in fruits and bioactive elements in juice, etc., so it is possible to solve it only in moving to the new level of ploidy